This paper deals with the topographic evaluation of samples made from materials applied in biomedicine. Different samples were made from Ti, TiAlV, TiNb and austenitic steel -Fe were used together with different surface morphology created. These samples were coated with a layer of TiNb alloy. The goal was to measure surface roughness in individual samples of biomaterials and to evaluate a 3D measurement of surface morphology made with a confocal microscope. Another task was to compare with appropriate software the pictures of material topography acquired at lower and higher magnification. Lower (10x) and higher (200x) magnification was used. For measured data evaluation SPIP and Gwyddion software were used. The same method of surface treatment was used but on different samples, which can lead to different results. The values obtained from topography measurements are crucial, but their formulation or evaluation can be affected by different processing methods. Our interest was to find and record the theoretically best procedure for the evaluation of measured data. Then to better understand the cell adhesion and integration of bacteria, we dealt with visualization of topography of given samples.

The article focuses on the issue of 3D measuring surface parameters using optical measuring devices, designing a statistical experiment and following evaluation. The development non-contact surface texture measurement methods lead to new possibilities for describing the surface of machine parts. But cannot be to rely only on the obtained the measured parameters values, and it is true that it is necessary to prepare the measurement. The processes of measurement enter many influences. Some of these effects can manage it, and then we call those factors. At the output of the receive process feedback. Method DOE (Design of Experiments) is used to locate a combination of factors that provide the most favorable response. The proposal described experiment compares and evaluates various surface roughness parameters of two different materials and machining technologies samples. Measurements were done by two operators, and each measurement was carried out three times. Measurement took place on a confocal laser microscope LEXT OLS 3000.