SummaryThe present study evaluates the acute toxicity of synthetic grape, plum and orange fl avourings in root meristem cells of Allium cepa at the doses of 3.5, 7.0 and 14.0 mL/kg and exposure times of 24 and 48 h, and in bone marrow erythrocytes of mice treated orally for seven days with 0.5, 1.0, 2.0, 5.0 and 10.0 mL/kg of fl avouring. The results of the plant test showed that grape, plum and orange fl avourings, at both exposure times, inhibited cell division and promoted the formation of a signifi cant number of micronuclei and mitotic spindle changes. These alterations were observed in at least one exposure time analysed, demonstrating a signifi cant cytotoxic, genotoxic and mutagenic activity. In mouse bioassay, animals treated with 2.0, 5.0 and 10.0 mL/kg of fl avouring died before the seventh day of treatment. The amounts of 0.5 and 1.0 mL/kg of the three additives were cytotoxic to erythrocytes, and treatment with the grape fl avouring signifi cantly induced the formation of micronucleated cells in the bone marrow of animals. Therefore, under the study conditions, the grape, plum and orange fl avouring additives promoted signifi cant toxicity to cells of the test systems used.