Objectives: Open access (OA) is based on a set of principles and a range of practices through which fruits of research are distributed online, free of cost, or other access barriers. According to the 2001 definition, OA publications are without barriers to copy or reuse with free access to readers. Some studies have reported higher rates of citation for OA publications. In this study, we analyzed the citation rates of OA and traditional nonOA (with or without free access) publications for authors publishing in the subspecialty of cytopathology during 2010–2015. Material and Methods: We observed and compared citation patterns for authors who had published in both OA and traditional non-OA, peer-reviewed, scientific, cytopathology journals. Thirty authors were randomly selected with criteria of publishing a total of at least five cytopathology articles over 2010–2015. Number of citations per article (CPA) (during 2010–2015) for OA publications (in CytoJournal and Journal of Cytology) and non-OA publications (in Diagnostic Cytopathology, Cytopathology, Acta Cytologica, Journal of American of Cytopathology, and Indian Journal of Pathology and Microbiology) was collected and compared statistically using two-tailed Student’s t-est. The data were collected manually through science citation analysis sites, mostly Google Scholar. Results: Thirty authors published 579 cytopathology articles in OA and non-OA journals. Average CPA for OA publications was 26.64. This was 11.35 higher than the average CPA) of non-OA conventional with subscription cytopathology journals (74% increase) and 11.76 higher than the average CPA of conventional cytopathology non-OA journal articles with free access (79% increase). These differences were statistically significantly with P < 0.05. Conclusion: We observed that the cytopathology publications in the OA journal attained a higher rate of CPA than the publications in the traditional non-OA journals in the field of cytopathology during 2010–2015.