This study was designed to determine the effect of acid and alkaline pretreatment of rice husk on the production of biosurfactant using Mucor indicus. Rice husk was pretreated at 120°C in a mineral nutrient medium at variable pH of 2, 7 and 12. The media were adjusted to pH7 after pretreatment to provide suitable environmental pH for microbial growth. Mucor indicus isolated from the rice husk dump site was used to inoculate the pretreated rice rusk media and the setup was allowed to stand for 28 days with intermittent shaking. At the expiration of 28 days, the broth was filtered and centrifuged to remove all suspended cells. The cell free broth was collected for the extraction of biosurfactant using a mixture of chloroform/methanol at ratio 2:1 (mixture/broth). The results revealed that the biosurfactants yield were 0.59±0.078, 0.40±0.042 and 0.78±0.050 g in 100 mL of broth for pH 2, 7 and 12 pretreated rice husk, respectively. The biosurfactants produced showed highest emulsification index for automotive gas oil and lowest for premium motor spirit. The results also suggested that the biosurfactants produced have glycolipids properties. Therefore, it will be correct to suggest that for the conversion of rice husk into useful material such as biosurfactant, the heat pretreatment should be carried out at an alkaline pH.