The composition of transgenic DAS-68416-4 soybean seed and forage was compared with that of non-transgenic soybean. DAS-68416-4 soybean expresses the aryloxyalkanoate dioxygenase-12 enzyme from the soil bacterium, Delftia acidovorans, which detoxifies 2,4-dichlorophenoxyacetic acid (2,4-D), conferring tolerance to this herbicide. DAS-68416-4 also expresses the phosphinothricin acetyltransferase enzyme from Streptomyces viridochromogenes which confers tolerance to glufosinate-ammonium herbicides. As expected for an input trait, results indicate that DAS-68416 soybean is compositionally equivalent to non-transgenic soybean. Methods and Materials Field phase Ten field experiments were conducted in 2009 to produce soybean forage and seed for compositional analysis. Field sites were located in Lonoke,

Event DAS-40278-9 maize expresses the aryloxyalkanoate dioxygenase-1 enzyme, which was originally identified in the soil bacterium Sphingobium herbicidovorans. This enzyme degrades 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate herbicides (e.g., haloxyfop, cyhalofop, quizalofop, etc.); therefore, plants that contain this enzyme are tolerant to these herbicides. We employed the substantial equivalence approach to investigate the compositional safety of event DAS-40278-9 maize. A total of 82 different compositional analyses were conducted to evaluate the equivalence of event DAS-40278-9 and conventional maize. Analyte levels within the transgenic entries were either within literature ranges for non-transgenic maize or statistically indistinguishable from the non-transgenic near-isogenic hybrid, thus indicating substantial equivalence between event DAS-40278-9 and its conventional counterpart. These results agree with dozens of published studies for other transgenic events where input traits were found to have a negligible effect on crop composition compared with traditional breeding methods.

DAS-81910-7 cotton is a transgenic event that was transformed to contain the aad-12 and pat genes. These genes code for the AAD-12 and PAT proteins, which confer tolerance to the herbicides 2,4-D and glufosinate, respectively. Crop composition studies were conducted with DAS-81910-7 cotton (both nonsprayed and sprayed with 2,4-D and glufosinate) to comply with requirements of regulatory authorities responsible for evaluating crop safety. Results indicate compositional equivalence between DAS-81910-7 cottonseed and nontransgenic cottonseed and between sprayed and nonsprayed DAS-81910-7 cottonseed. This study builds on the results from many prior studies which support the conclusion that transgenesis is less likely to unexpectedly alter the composition of crops as compared with traditional breeding.

Field desorption production of protonated molecular ions from nanomole amounts of chemically underivatized penta-, tetra-and dipeptides, coupled with collision induced decomposition of [M + HI' ions, yielded partial amino acid sequence information. Linked scanning of two of the three fields detected ions produced in the first field free region of a double focusing mass spectrometer.