We compared results of the stannous chloride and vanadate methods for estimating serum inorganic phosphorus on the "SMA 12/60" (Technicon). The technically simpler vanadate method gave better linearity and reproducibility, with virtually no carry-over or baseline drift; the reagents were stable, and it was most important to ensure that the concentration of sulfuric acid in the recipient and sample streams was identical. Instability in the stannous chloride method was due to precipitation in the stannous chloride-hydrazine working solution.
In order to evaluate the effectiveness of a structured respiratory teaching programme, clients with chronic obstructive pulmonary disease who were hospitalized in a 13-month period both prior to and following the implementation of the programme were compared on several parameters to assess changes in their ability to manage their disabilities. The study demonstrated that clients who participated in the teaching sessions had fewer hospital admissions, shorter lengths of hospital stay, and longer stays in the community. A sub-group of clients who were or had been in at-risk occupations was identified as representing the 'revolving door' client with shorter hospital and community stays. The teaching programme is described as well as specific elements that can be used for a shorter teaching experience to respond to time constraints when necessary.
The in vitro stability of creatine kinase isoforms was examined by separation with high voltage electrophoresis. The effect of inhibitors of carboxypeptidase was evaluated. Preservation of samples is essential to inhibit in vitro changes in isoform pattern. EDTA at a final concentration of 15 mmol/L is recommended. Using appropriately preserved samples, normal reference intervals for the MM isoforms have been established.
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