Summary Reverse genetics approaches have revolutionized plant biology and agriculture. Phenomics has the prospect of bridging plant phenotypes with genes, including transgenes, to transform agricultural fields. Genetically encoded fluorescent proteins (FPs) have revolutionized plant biology paradigms in gene expression, protein trafficking and plant physiology. While the first instance of plant canopy imaging of green fluorescent protein (GFP) was performed over 25 years ago, modern phenomics has largely ignored fluorescence as a transgene expression device despite the burgeoning FP colour palette available to plant biologists. Here, we show a new platform for stand‐off imaging of plant canopies expressing a wide variety of FP genes. The platform—the fluorescence‐inducing laser projector (FILP)—uses an ultra‐low‐noise camera to image a scene illuminated by compact diode lasers of various colours, coupled with emission filters to resolve individual FPs, to phenotype transgenic plants expressing FP genes. Each of the 20 FPs screened in plants were imaged at >3 m using FILP in a laboratory‐based laser range. We also show that pairs of co‐expressed fluorescence proteins can be imaged in canopies. The FILP system enabled a rapid synthetic promoter screen: starting from 2000 synthetic promoters transfected into protoplasts to FILP‐imaged agroinfiltrated Nicotiana benthamiana plants in a matter of weeks, which was useful to characterize a water stress‐inducible synthetic promoter. FILP canopy imaging was also accomplished for stably transformed GFP potato and in a split‐GFP assay, which illustrates the flexibility of the instrument for analysing fluorescence signals in plant canopies.

Spontaneous imbibition (SI) is a capillary-driven flow process, in which a wetting fluid moves into a porous medium displacing an existing non-wetting fluid. This process likely contributes to the loss of fracking fluids during hydraulic fracturing operations. It has also been proposed as a method for an enhanced recovery of hydrocarbons from fractured unconventional reservoirs. Numerous analytical and numerical approaches have been employed to model SI. Invariably, these idealize a fracture as the gap formed between parallel flat surfaces. In reality, rock fracture surfaces are rough over multiple scales, and this roughness will influence the contact angle and rate of fluid uptake. We derived an analytical model for the early-time SI behavior within a fracture bounded by parallel impermeable surfaces with fractal roughness assuming laminar flow. The model was tested by fitting it to experimental data for the SI of deionized water into air-filled rock fractures. Twenty cores from two rock types were investigated: a tight sandstone (Crossville) and a gas shale (Mancos). A simple Mode I longitudinal fracture was produced in each core by compressive loading between parallel flat plates using the Brazilian method. Half of the Mancos cores were fractured perpendicular to bedding, while the other half were fractured parallel to bedding. The two main parameters in the SI model are the mean separation distance between the fracture surfaces, [Formula: see text], and the fracture surface fractal dimension [Formula: see text]. The [Formula: see text] was estimated for each core by measuring the geometric mean fracture aperture width through image analysis of the top and bottom faces, while [Formula: see text] was estimated inversely by fitting the SI model to measurements of water uptake obtained using dynamic neutron radiography. The [Formula: see text] values ranged from 45[Formula: see text][Formula: see text]m to 190[Formula: see text][Formula: see text]m, with a median of 93[Formula: see text][Formula: see text]m. The SI model fitted the height of uptake versus time data very well for all of the rock cores investigated; medians of the resulting root mean squared errors and coefficients of determination were 0.99[Formula: see text]mm and 0.963, respectively. Estimates of [Formula: see text] ranged from 2.04 to 2.45, with a median of 2.24. Statistically, all of the [Formula: see text] values were significantly greater than two, confirming the fractal nature of the fracture surfaces. Future research should focus on forward prediction through independent measurements of [Formula: see text] and extension of the existing SI model to late times (through the inclusion of gravity) and fractures with permeable surfaces.

No abstract

Core Ideas Spontaneous imbibition was measured in rock fractures using neutron radiography. Early‐time uptake of water displacing air showed a square root of time dependency. Fracture sorptivity was quantified from the slope of the early‐time uptake data. Fracture sorptivity increased with increasing fracture aperture width. Fracture sorptivity decreased with increasing fracture surface roughness. Fractures in low‐porosity rocks can provide conduits for fluid flow. Numerous researchers have investigated fluid flow through fractures under saturated conditions. However, relatively little information exists on spontaneous imbibition in fractures, whereby a wetting fluid displaces a non‐wetting fluid by capillarity. We investigated spontaneous imbibition of water displacing air in a suite of fractured low‐porosity sedimentary and igneous rock cores (5.08‐cm length by 2.54‐cm diameter). Mode I fractures were induced in the cores by compression between opposing parallel flat plates. The following physical properties were measured: bulk density, ρb; solid‐phase density, ρs; porosity, ϕ; contact angle, θe; fracture aperture width, xgeo; and fracture surface roughness, Wr. The wetting front in each fracture was imaged using dynamic neutron radiography. Early‐time uptake exhibited a square root of time dependency, and was quantified by linear regression, with the slope equal to the fracture sorptivity, Sf. Estimates of Sf ranged from 10.1 to 40.5 mm s−0.5, with a median value of 25.0 mm s−0.5. There was a statistically significant effect of rock type on Sf, with igneous rocks generally having lower mean values than sedimentary rocks. Differences in ρb, ρs, ϕ, and θe between the rock types did not contribute significantly to the variation in Sf. However, xgeo and Wr were significantly correlated with Sf. These correlations indicated that Sf increases with increasing xgeo, as predicted by early‐time capillary theory, and decreases with increasing Wr, analogous to the decrease in fracture permeability with increasing surface roughness observed under saturated flow conditions.

No abstract