Melanoeytes and melanoma cells are known to possess receptors for melanoeyte stimulating hormone (MSH). A eDNA clone, designated l ID, has b~.~n isolated from human melanoma cells and encodes a MSH receptor. The cloned eDNA encodes a 317 amino acid protein with transmembrane topography characteristics of a G-protein.coupled receptor, bat it does not show striking similarity to already pablish~ sequences of other G-protein-coupl~ receptors. When 11D eDNA is expressed in COS-7 cells, it binds an Z~l-labelled MSH analogue (NDP-MSH) in a Sl~Cifie manner. The bound ligand could be displaced by melanotropie peptides, a~-MSH, fl.MSH, 7-MSH and ACTH (adrenoeonieotropie hormone), but not by the non-melanotropie ~ptid¢,/3-endorphine. This is the first report of the cloning of a receptor geae of the melanotropin receptor family.

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