ETV6 gene abnormalities are well described in tumor pathology. Many fusion partners of ETV6 have been reported in a variety of epithelial, mesenchymal, and hematological malignancies. In salivary gland tumor pathology, however, the ETV6-NTRK3 translocation is specific for (mammary analog) secretory carcinoma, and has not been documented in any other salivary tumor type. The present study comprised a clinical, histologic, and molecular analysis of 10 cases of secretory carcinoma, with typical morphology and immunoprofile harboring a novel ETV6-RET translocation.

Abstract. Potential involvement of circadian clock genes in so far unknown mechanism of photoperiodic time measurement is an important question of insect life-cycle regulation science. Here we report about the cloning of full-length cDNA of the structural homologue of the Drosophila's timeless gene in Chymomyza costata. Its expression was compared in two strains: a wild-type strain, responding to short days by entering larval diapause and a «pd-mutant strain, showing no photoperiodic response. The timeless mRNA transcripts were not detectable by Northern blot analysis in the fly heads of «pd-mutants, while they were detectable and showed typical daily oscillations in the wild-type strain. After disrupting the normal process of timeless transcription in the wild-type strain by injection of timeless double-strandRNA into early embryos of wild-type (RNAi method: Kennerdell & Carthew 1998, 2000, a certain proportion of the individuals adopted a «pd-mutant phenotype, showing no-diapause in response to short-daylength. Cloning of genomic DNA fragments revealed that «pd-mutants carry a different allele, timeless"1 "1, with a 13-bp insertion in an intron positioned within the 5'-leader sequence. Genetic linkage analysis showed that the 13-bp insertion (a marker for timeless"1 "1) and the absence of response to short days (a marker for «pd-phenotype) are strictly co-inherited in the F2 progeny of the reciprocal crosses between wild-type and «pd-mutant flies. Such results indicated that the locus «pd could code for the timeless gene in C. costata and its product might thus represent a molecular link between circadian and photoperiodic clock systems in this fly.

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