The following paper includes research about ergonomics of e-commerce web applications. Main purpose of experiment was to compare existing application of Morele.net shop and developed prototype of application using eyetracking examination and survey. The study carried out on a group of 40 students provided heat maps, scan paths, number of fixations and saccades, times to the first fixation in area of interest, task completion times, assessments of both applications in the form of WUP indicators. Based on the qualitative and quantitative analysis, conclusions were drawn confirming the hypothesis put forward in the work that there is an impact of ergonomic placement of navigation elements on the accessibility and usability of the application, as well as the time of performing tasks in it.