Background:The conserved Q motif of RNA/DNA helicases has been structurally implicated in nucleotide binding; however, its biochemical functions are less certain. Results: Mutagenesis of the Q motif in FANCJ DNA helicase perturbs dimerization, DNA binding, and catalytic function. Conclusion: The Q motif is essential for FANCJ enzymatic activity and DNA repair. Significance: The FANCJ Q motif is important for its biochemical and cellular functions.
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