BACKGROUND Wild pigs (Sus scrofa) are a destructive invasive species throughout many regions of the world. In 2018, a field evaluation of an early prototype of a sodium nitrite (SN) toxic bait in the United States revealed wild pigs dropped large amounts of the toxic bait outside the pig‐specific bait stations while feeding, and thus subsequent hazards for non‐target animals. We modified the SN‐toxic bait formulation, the design of the bait station, and the baiting strategy to reduce dropped bait. We tested the modifications in Queensland, Australia (December 2018), Alabama, USA (August 2019), and Texas, USA (March 2020) under differing climatic and seasonal conditions for one night. RESULTS Cumulatively we found 161 carcasses of all age classes of wild pigs using systematic transects. Remote camera indices indicated high lethality for wild pigs, achieving population reductions of 76.3 to 90.4%. Wild pigs dropped only small particles of SN‐toxic bait (average = 55.5 g per bait site), which represented a 19‐fold decrease from the previous trial. Despite this reduction, we found three Australian ravens (Corvus coronoides) in Queensland, two Virginia opossums (Didelphis virginiana) in Alabama, and 35 granivorous‐passerine birds (mostly dark‐eyed juncos [Junco hyemalis]) in Texas dead from consuming the dropped bait. We did not detect any population‐level effects for those species. CONCLUSION Our modifications were effective at reducing populations of wild pigs, but the deaths of non‐target species require further steps to minimize these hazards. Next steps will include evaluating various deterrent devices for birds the morning after SN‐toxic bait has been offered. Published 2020. This article is a U.S. Government work and is in the public domain in the USA

Abstract. An electrophoretic survey of allozyme variation revealed substantial genetic differentiation within the eastern Australian population of Actinia tenebrosa. This differentiation appears to reflect the effects of both asexual reproduction and limited gene flow among local populations separated by up to 1050 km. Variation was assessed within groups of 27 to 55 adults sampled between September 1985 and December 1988 collected from small areas of shore within each of 24 local populations. All individuals were collected from stable rock platforms, with the exception of Boulder Bay, where some sea anemones were removed from small mobile boulders. High levels of variability were detected for each of seven enzyme-encoding loci. The patterns of genotypic variation detected imply that local populations are maintained by predominantly asexually generated recruitment. Levels of multi-locus genotypic diversity within samples were consistently less than 50 % of the level expected for sexual reproduction with free recombination. This was reflected by the detection of relatively low numbers of multi-locus genotypes and significant departures from expectations for single-locus Hardy-Weinberg equilibria within 17 of the 24 local populations. Standardised genetic variances (Fsr), calculated from the genotypes of all individual adults were typically much greater than those expected for marine organisms with widely dispersed larvae. The former values were reduced, but were still extremely large when "clonal" genotype frequencies were substituted into the calculation.

Context Feral pigs (Sus scrofa) are a destructive invasive species that cause damage to ecologically sensitive areas. Management of biodiversity and of feral pigs assumes the diet of pigs of different ages and sexes are similar. Aims We aimed to investigate effects of feral pig age and sex on broad feral pig diet to identify potential at-risk native wildlife species so as to improve biodiversity and feral pig management. Methods Diet was determined by macroscopic analysis of the stomach content of 58 aerially shot feral pigs of mixed ages and sexes. The study occurred in the Macquarie Marshes, New South Wales, a Ramsar wetland of international significance. Results Feral pigs were largely herbivorous, with vegetable matter being found in all stomachs and contributing to a majority of the food material that was present in each stomach, by volume. Adult feral pigs had significantly more grasses and crop material in their stomachs than juveniles, while juveniles had significantly more forbs in their stomachs than adult feral pigs. Vertebrate prey items included frogs, lizard and snake, but no threatened wildlife species. Conclusions Juvenile and adult feral pigs differed in their diet, especially with regards to plant material, which has not been reported previously. There was, however, no difference in the consumption of vertebrate wildlife species between juvenile and adult, or male and female feral pigs. Slow-moving, nocturnal amphibians and reptiles were the most common vertebrate item recorded. Implications Biodiversity and feral pig management should recognise plant diet differences between demographic segments of the feral pig population. Further research is recommended to determine if diet differences also occur for threatened wildlife species, which will require more intensive nocturnal sampling.

Context Wildlife and pest managers and stakeholders should constantly aim to improve animal-welfare outcomes when foot-hold trapping pest animals. To minimise stress and trauma to trapped animals, traps should be checked at least once every 24h, normally as soon after sunrise as possible. If distance, time, environmental or geographical constraints prevent this, toxins such as strychnine can be fitted to trap jaws to induce euthanasia. However, strychnine is considered to have undesirable animal-welfare outcomes because animals are conscious while clinical signs of intoxication are present. A toxin considered more humane, para-aminopropiophenone (PAPP), is available to induce euthanasia in trapped animals but is untested for presentation and efficacy. Aim We tested the efficacy of two types of lethal trap device (LTD’s), each using a paste formulation of PAPP as the active toxin to replace the use of strychnine on foot-hold jaw traps. Methods Elastomer LTDs and PAPP-cloths were fitted to jaw traps set to capture wild dogs (Canis familiaris). Camera-trap data was used to record animal behaviours after capture and to determine the efficacy of both modalities. Key results Every trapped wild dog (n=117) gnawed at the elastomer LTD’s or PAPP-cloth attached to the trap jaws that restrained them; one dog failed to liberate the toxin. From the dogs caught in the main trial (n=56), a mortality rate of 84% and 87% was reported respectively. The mean time from trap-to-death for elastomer LTDs was 64min and 68min for PAPP-cloths. Conclusions Elastomer LTDs and PAPP cloths combined caused the mortality of 85% of captured dogs. This efficacy could be improved by adopting the recommendations discussed in the present study for deploying PAPP-based LTDs during trap deployment. Implications PAPP-based LTDs offer an alternative option to the use of strychnine and improve the welfare outcomes for trapped predators, especially where traps are not checked within the recommended 24-h period.

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