Because of the increasing number of glyphosate-resistant weeds, alternate herbicide-resistant crops and herbicides with different modes of action are required to protect crop yield. Glufosinate is a broad-spectrum POST herbicide for weed control in glufosinate-resistant crops, including soybean. The objective of this study was to compare herbicide programs with glufosinate applied singly at late-POST (LPOST) or sequentially at early POST (EPOST) followed by (fb) LPOST applications and PRE herbicides fb EPOST/LPOST glufosinate alone or tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor in glufosinate-resistant soybean. A field experiment was conducted at the South Central Agriculture Laboratory in Clay Center, NE, in 2012 and 2013. Glufosinate applied in a single LPOST or sequential EPOST fb LPOST application controlled common lambsquarters, common waterhemp, eastern black nightshade, green foxtail, large crabgrass, and velvetleaf 82% and resulted in a weed density of 6 to 10 plants m À2 by the end of the season. Flumioxazin-, saflufenacil-, or sulfentrazone-based premixes provided 84 to 99% control of broadleaf and grass weeds tested in this study at 15 d after PRE application and a subsequent LPOST application of glufosinate alone controlled broadleaf and grass weeds 69 to 93% at harvest, depending on the herbicide program and weed species being investigated. The PRE application of sulfentrazone plus metribuzin fb EPOST glufosinate tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor controlled the tested broadleaf and grass weeds ! 90%, reduced density to 2 plants m À2 , and reduced weed biomass to 10 g m À2 and produced soybean yields of ! 4,450 and 3,040 kg ha À1 in 2012 and 2013, respectively. Soybean injury was 0 to 20% from PRE or POST herbicides, or both and was inconsistent, but transient, during the 2-yr study, and it did not affect soybean yield. Sulfentrazone plus metribuzin applied PRE fb glufosinate EPOST tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor provided the highest level of weed control throughout the growing season and increased soybean yield compared with a single LPOST or a sequential EPOST fb LPOST glufosinate application. Additionally, these herbicide programs provide four distinct mechanisms of action that constitute an effective weed-resistance management strategy in glufosinateresistant soybean. Nomenclature: Acetochlor; flumioxazin; glufosinate; metribuzin; pyroxasulfone; saflufenacil; Smetolachlor; sulfentrazone; common lambsquarters, Chenopodium album L.; common waterhemp, Amaranthus rudis Sauer.; eastern black nightshade, Solanum ptychanthum Dunal; green foxtail, Setaria viridis (L.) Beauv.; large crabgrass, Digitaria sanguinalis (L.) Scop.; velvetleaf, Abutilon theophrasti Medik.; soybean, Glycine max (L.) Merr.Debido al creciente número de malezas resistentes a glyphosate, es necesario alternar cultivos resistente a herbicidas con diferentes modos de acción para proteger los rendimientos de los cultivos. Glufosinate es un herbicida POST de amplio esp...

Glyphosate-resistant common waterhemp is a difficult-to-control annual broadleaf weed that has become a serious management challenge for growers in Nebraska and other states in the United States. The objectives of this study were to confirm glyphosate-resistant common waterhemp in Nebraska by quantifying level of resistance in a dose-response study, and to determine the sensitivity and efficacy of POST soybean herbicides for controlling suspected glyphosate-resistant common waterhemp biotypes. Seeds of suspected glyphosate-resistant common waterhemp biotypes were collected from seven eastern Nebraska counties. Greenhouse dose-response experiments were conducted to evaluate the response of common waterhemp biotypes to nine rates of glyphosate (0 to 16×). Common waterhemp biotypes were 3- to 39-fold resistant to glyphosate depending on the biotype being investigated and the susceptible biotype used for comparison. Results of the POST soybean herbicides efficacy experiment suggested that glyphosate-resistant biotypes, except a biotype from Pawnee County, had reduced sensitivity to acetolactate synthase (ALS)–inhibiting herbicides (chlorimuron-ethyl, imazamox, imazaquin, imazethapyr, and thifensulfuron-methyl). Glufosinate and protoporphyrinogen oxidase (PPO)–inhibiting herbicides (acifluorfen, fluthiacet-methyl, fomesafen, and lactofen) provided ≥ 80% control of glyphosate-resistant common waterhemp at 21 d after treatment (DAT). This study confirmed the first occurrence of glyphosate-resistant common waterhemp in Nebraska, and also revealed reduced sensitivity to ALS-inhibiting herbicides in most of the biotypes tested in this study.

Palmer amaranth, a dioecious summer annual species, is one of the most troublesome weeds in the agronomic crop production systems in the United States. In the last two decades, continuous reliance on herbicide s with the same mode of action as the sole weed management strategy has resulted in the evolution of herbicideresistant HR weeds, including Palmer amaranth. By , Palmer amaranth biotypes had been confirmed resistant to acetolactate synthase ALS -inhibitors, dinitroanilines, glyphosate, hydroxyphenylpyruvate dioxygenase HPPD -inhibitors, and triazine herbicides in some parts of the United States along with multiple HR biotypes. Mechanisms of herbicide-resistance in Palmer amaranth are discussed in this chapter. Preplant herbicide options including glufosinate, , -D, and dicamba provide excellent Palmer amaranth control however, their application is limited before planting crops, which is often not possible due to unfavorable weather conditions. Agricultural biotechnology companies are developing new multiple HR crops that will allow the post-emergence application of respective herbicides for management of HR weeds, including Palmer amaranth. For the effective in-crop management of Palmer amaranth, and to reduce the potential for the evolution of other HR weeds, growers should apply herbicides with different modes of action in tank-mixture and should also incorporate cultural practices including inversion tillage and cover crops along with herbicide programs.

A three year field experiment was conducted to evaluate the role of soil-inversion, cover crops and herbicide regimes for Palmer amaranth between-row (BR) and within-row (WR) management in glufosinate-resistant cotton. The main plots were two soil-inversion treatments: fall inversion tillage (IT) and non-inversion tillage (NIT). The subplots were three cover crop treatments: crimson clover, cereal rye and winter fallow; and sub subplots were four herbicide regimes: preemergence (PRE) alone, postemergence (POST) alone, PRE + POST and a no herbicide check (None). The PRE herbicide regime consisted of a single application of pendimethalin at 0.84 kg ae ha −1 plus fomesafen at 0.28 kg ai ha −1 . The POST herbicide regime consisted of a single application of glufosinate at 0.60 kg ai ha −1 plus S-metolachlor at 0.54 kg ai ha −1 and the PRE + POST regime combined the prior two components. At 2 weeks after planting (WAP) cotton, Palmer amaranth densities, both BR and WR, were reduced ≥90% following all cover crop treatments in the IT. In the NIT, crimson clover reduced Palmer amaranth densities >65% and 50% compared to winter fallow and cereal rye covers, respectively. At 6 WAP, the PRE and PRE + POST herbicide regimes in both IT and NIT reduced BR and WR Palmer amaranth densities >96% over the three years. Additionally, the BR density was reduced OPEN ACCESSAgronomy 2012, 2 296≥59% in no-herbicide (None) following either cereal rye or crimson clover when compared to no-herbicide in the winter fallow. In IT, PRE, POST and PRE + POST herbicide regimes controlled Palmer amaranth >95% 6 WAP. In NIT, Palmer amaranth was controlled ≥79% in PRE and ≥95% in PRE + POST herbicide regimes over three years. POST herbicide regime following NIT was not very consistent. Averaged across three years, Palmer amaranth controlled ≥94% in PRE and PRE + POST herbicide regimes regardless of cover crop. Herbicide regime effect on cotton yield was highly significant; the maximum cotton yield was produced by the PRE + POST herbicide regime. Averaged over three years, the PRE, POST and PRE + POST cotton yields were about three times higher than no herbicide regime. In a conservation tillage production system, a PRE + glufosinate POST herbicide based regime coupled with a cereal rye cover crop may effectively control Palmer amaranth and maximize cotton yields.

With the intent to control glyphosate-resistant and hard to control weeds, a formulation of 2,4-D choline (24.4%) and glyphosate (22.1%) (Enlist Duo™ herbicide) (Note 1) has been developed recently to be used post-emergence in corn and soybean tolerant to Enlist Duo™ in the United States. Dose response studies were conducted under greenhouse conditions for the evaluation of effective rates of Enlist Duo™ to control glyphosate-resistant common waterhemp (Amaranths rudis Sauer), giant ragweed (Ambrosia trifida L.), and kochia [Kochia scoparia (L.) Schrad] and to determine the effect of growth stage of these weeds on the efficacy of Enlist Duo™. Three parameter log-logistic models were used to develop dose response curves. Glyphosate-resistant giant ragweed was the most sensitive of the three weed species, followed by common waterhemp, and kochia. Based on the visual control or injury estimates, the Enlist Duo™ rates required for 90% control (ED 90 ) of common waterhemp, giant ragweed, and kochia were 1179, 825, and 4,382 g ae ha -1 , respectively, for 10-cm tall plants compared to 2,480, 1,101, and 5,305 g ae ha -1 , respectively, for 20-cm tall plants at 21 days after treatment (DAT). The ED 90 values calculated on the basis of percent shoot biomass reduction and visual control or injury estimates were usually similar at 21 DAT. The greenhouse studies indicate that Enlist Duo™ can effectively control less than or equal to 20-cm tall glyphosate-resistant giant ragweed and less than or equal to 10-cm tall glyphosate-resistant common waterhemp at the recommended rate (1,640 g ae ha -1 ).