The multi-country outbreak of monkeypox virus (MPXV) infection, while the coronavirus disease 2019 pandemic is still an ongoing issue, has caused a new challenge.The re-emergence of MPXV and the rising incidence in non-endemic countries is turning into an upcoming threat to global health. Hence, rapid identification of the virus with appropriate methodology with the lowest false results plays a critical role in estimating the global extent of the crisis and providing preventive measures. This review summarised the main applicable strategies for primary detection and confirmation of MPXV and highlighted available data in biosafety, requirements, standard operating procedures, specimen collection, transportation and storage of clinical samples, and waste disposal of the viral agent. Also, various assays including molecular techniques, immunoassays, histopathological methods, electron microscopy, genomic sequencing, and cell culture have been illustrated. Moreover, we reflected on current knowledge of the advantages and disadvantages of each approach.

Summary Human tumor viruses are either casually linked or contribute in the development of human cancers. Viruses can stimulate oncogenesis through affecting diverse biological pathways in human cells. Growing data have demonstrated frequent involvement of one of the most characteristic parts of cellular epigenetic machinery, DNA methylation, in the oncogenesis. DNA methylation of cellular genes is catalyzed by DNA methyltransferases (DNMTs) as a key effector enzyme in this process. Dysregulation of DNMTs can cause aberrant gene methylation in promoter of cancer‐related genes including tumor suppressor genes, resulting in gene silencing. In this regard, the role of tumor viruses is remarkable. Here, in this review, we used published information to elucidate whether tumor viruses are able to manipulate DNMT regulation, and if so, what are its consequences in the process of oncogenesis. This essay also aims to shed light on which cellular pathways have been engaged by viruses to induce DNMTs.

Among the DNA tumor viruses Epstein-Barr virus (EBV) and Kaposi sarcoma herpesvirus (KSHV), account for a considerable percentage of virus-associated cancers. Deregulation of transcription factors signaling pathways is one of the most significant oncogenic characteristics of EBV and KSHV. NF-κB is a transcription factor that play a remarkable role in oncogenesis because of its function as a master regulator of a spectrum of genes involved in physiological and pathophysiological process. Constitutive activation of NF-κB is a frequent and well-described event in many human malignancies. Compelling evidence represent EBV and KSHV are capable of targeting different components of NF-κB cascade. Here, we summarized recent findings to clarify the precise relationship between dysregulation of NF-κB and EBV and KSHV-related malignancies. This essay also emphasizes on contribution of various viral products in developing cancer through alteration of NF-κB signaling pathway.

Vaccination has had great success in history of medicine with both infectious and non-infectious diseases listed in the realm of vaccines. The significant decrease in deaths from infectious and non-infectious diseases by development of vaccines has had a huge impact on the world health. Since the 20th century, for preventive and treatment purposes, there has been an emergence of new and diverse range of vaccines in medicine. Also, there are ongoing extensive efforts to increase the efficiency and performance of vaccines and reduce the risks, which are associated with humans. Despite of the development of advanced and effective vaccines in the recent decades, vaccines should be presented with different mechanisms to prevent epidemic and pandemic diseases. Vaccination has received the greatest attention in military aspects and several vaccines have been developed. Therefore, it seems that the study of different types and generations of vaccines, understanding their evolution, and production and development of new and modern vaccine is essential.

Background Blood group antigens are one of the most important antigens in humans that have an impact on susceptibility to disease and may be used as a prognosis factor in different diseases such as covid-19. Objectives Study aimed to investigate the relationship between ABO blood groups and Rhesus antigen and susceptibility to COVID-19. Methods The clinical data of 398 subjects were used in the investigation collected from 148 cases vs. 250 controls. This information was obtained from Shahid Sadoughi Hospital of Yazd (IRAN) University. blood groups and outcomes was assessed using statistical tests for four populations: COV + vs. COV− and COV +/deceased vs. COV +/live. Results Out of a total of 148 COVID19 patients, 80 (54/1%) were male, 68 (45/9%) were female. Among these patients 33 (22/6%) had type A+, 44 (30/1%) had type B+, 13 (8/9%) had type AB+ and 36 (24/7%) had type O+. On the other hand, out of a 148 patients, 126 (86/3%) had positive blood types and 20 (13/7%) had negative blood types. As a result, no significant difference was found in the relationship between ABO blood groups and RH type and susceptibility to COVID-19 (p-value= 0.392 and p-value=0.847, respectively). Other data showed a significant difference between patients group with other parameters such as age (p-value<0.001) and gender (p-value<0.001). Conclusion Although in this study there was no association between blood type and RH type with COVID-19, findings about association between age and gender can confirm the results of previous studies.