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Direct ink writing (DIW) belongs to extrusion-based 3D printing techniques. The success of DIW process depends on well-printable ink and optimized process parameters. After ink preparation, DIW process parameters considerably affect the parts' dimensional accuracy and process parameters optimization for dimensional accuracy of printed layers is necessary for quality control of parts in DIW. In this study, DIW process parameters were identified and divided into two categories as the parameters for printing a line and the parameter from lines to a layer. Then a two-step method was proposed for optimizing process parameters.Step 1 was to optimize process parameters for printing a line. In Step 1, continuity and uniformity of extruded filaments and printed rectangular objects were observed in screening experiments to determine printability windows for each process parameter. Then, interaction effect tests were conducted and degree of freedom (DOF) for experiments was calculated followed by orthogonal array (OA) selection for Taguchi design. Next, main experiments of line printing based on Taguchi method were conducted. Signal-to-noise ratio (SNR) calculations and analysis of variance (ANOVA) were performed to find the optimal combination and evaluate the significance respectively. Step 2 was to optimize the parameter from lines to a layer. InStep 2, the average width of the printed line under optimal condition was measured firstly. Then, single-factor tests of rectangular object printing were conducted to find the optimal parameter from lines to a layer. After these two steps, confirmation results were conducted to verify the reliability of the proposed method and the method robustness on other shapes and other materials; parameter adaptability in 3D parts printing from printed layers' analyses for the proposed method and parameter adaptability in constructs fabricated as 100% infill or with porosities.