Lignocellulosic biomass is recalcitrant due to its heterogeneous structure, which is one of the major limitations for its use as a feedstock for methane production. Although different pretreatment methods are being used, intermediaries formed are known to show adverse effect on microorganisms involved in methane formation. This review, apart from highlighting the efficiency and limitations of the different pretreatment methods from engineering, chemical, and biochemical point of views, will discuss the strategies to increase the carbon recovery in the form of methane by way of amending pretreatments to lower inhibitory effects on microbial groups and by optimizing process conditions.

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