BACKGROUND Objectives-1). To assess the correlation between Transcutaneous Bilirubinometer readings (TCB) at the sternum with Total Serum Bilirubin (TSB) levels in evaluating jaundice in newborns > 32 weeks of gestation cared in the Newborn nursery at Govt. T. D. Medical College, Alappuzha, during a period of 1 year. 2). To assess the correlation between Transcutaneous Bilirubinometer readings at different sites (Forehead, sternum and pubic symphysis) with serum bilirubin levels. MATERIALS AND METHODS 160 babies > 32 weeks of gestation cared in the newborn nursery of Govt. T.D. Medical College Alappuzha, with clinical jaundice at least up to thighs were selected for the study. Infants with icterus from zone 3 onwards were selected. Sick babies, babies born through meconium stained amniotic fluid, babies with hepatic dysfunction and congenital anomalies were excluded. Transcutaneous bilirubinometry was done at forehead, sternum and pubic symphysis. Blood for total serum bilirubin was taken within 10 minutes of performing transcutaneous bilirubinometry. RESULTS There is a positive correlation between TCB (at sternum) with serum TSB (r= 0.669, p value < 0.001). The linear regression equation is TCB= 6.371 + (0.581 X TSB). Maximum correlation is found between TCB values at forehead (r= 0.722, p value < 0.001) and TSB values compared to those at sternum (r= 0.669) and pubic symphysis (r= 0.591). Positive correlation between entire range of TCB with TSB was also found, but was maximum when TSB is < 10 mg/dL (r= 0.625). The sensitivity and specificity of TCB is 80.7% and 79.6%, respectively. CONCLUSION Transcutaneous bilirubinometer can be used as a screening tool for non-invasive assessment of neonatal jaundice. The best site for transcutaneous bilirubinometry is forehead.