The effects of variety and tapping length on several physical, biochemical, nutritional and microbiological parameters (viscosity, pH, Total Titratable Acidity (TTA), fermenting microorganisms, contaminants) of palm wine extracted from two varieties of palm oil tree (Dura and Tenera) were studied. Each variety presented its own palm wine specific characteristics regarding the chosen parameters. Microbiological and biochemical contents of palm wine were determined during the tapping of Dura and Tenera felled oil palm trees for 4 weeks. Some differences in chemical compositions between fresh palm wine samples of two palm trees varieties were observed. The exudates obtained during the first day of tapping of Dura palm wine were very sugary, less sour and did not contain substantial concentrations of alcohol but the highest loads of microorganisms were observed in Tenera palm wine. Throughout the tapping of palm wine, yeasts and lactic acid bacteria population changed with undoubtedly influence on the palm wine quality.
After previously showing rhizobacteria Pseudomonas fluorescence and foliar bio-fertilizer mixture as a suitable practice in improving maize and soybean crops development and dry biomass, this study thus embark on predicting the above-mentioned plants dry biomass feature through their yield and growth parameters by assessing P. fluorescence activities on foliar bio-fertilizer in an arid agro-climate. For this purpose, several scripts and functions from our previous computational statistic pipeline, developed in R programming environment were partially used for statistical survey. Several multiple linear regression models have been achieved aimed at understanding the links between maize and soybean crops (i) dry biomass, (ii) yield and (iii) growth features as well as highlights the synergic roles of rhizobacteria microorganisms and foliar bio-fertilizer on that relationship. Findings, revealed as expected, a significant difference in term of crops development between plants from seeds without treatment and those from seeds under (i) rhizobacteria, (ii) rhizobacteri + foliar bio-fertilizer mixture and (iii) foliar biofertilizer treatments (p<0.05). Also, yield parameters exhibited a substantial variability reacting to rhizobacteria microorganism as well as to rhizobacteria microorganism + foliar bio-fertilizer mixture as opposed to growth features. Hence, the present results clearly showed significant difference between rhizobacteria microorganism and foliar bio-fertilizer activities impacting both maize and soybean crops yield and growth features (p<0.05). Indeed, findings suggested a selective impact of rhizobacteria microorganisms (P. fluorescence) on processed plants yield features. The same findings exhibited P. fluorescence and foliar bio-fertilizer mixture, as adjusting the synergy between both processed crops growth and yield parameters by improving dry biomass feature (p= 0.17). Finally, this study emphasized rhizobacteria microorganism's activities on foliar bio-fertilizer in an arid agro-climate low fertile soil as a weighty promoter improving maize and soybean crops dry biomass by normalizing the relationship concerning growth and yield features.Key words: Rhizobacteria (Pseudomonas fluorescence), growth and yield parameters, dry biomass, maize (Zea mays. L.) and soybean (Glycine max), computational statistic.
Background: Morinda morindoides (Baker) (Rubiaceae) is a medicinal plant with antimicrobial properties currently used in Côte d'Ivoire and other countries. These properties have been described but most of the studies are dealing with crude extracts. Objective: The chemical structures of the bioactive compounds extracted from Morinda morindoides roots have been characterized. Methods: The root extracts were analyzed by using HPLC. Fourteen fractions were detected among which 11 compounds have been structurally identified by using a combination of 1H-NMR and 13CNMR and LC-HRMS methodologies. Results: All these compounds belong to anthraquinone family. The antibacterial activity of the eleven compounds was tested against six strains of microorganisms with ofloxacin as standard (two Gram-negative bacteria, two Gram-positive bacteria and two yeasts). The Minimal Inhibitory Concentrations recorded, varies from 8 to 128 μg / ml. Staphylococcus aureus was the most susceptible organism. Conclusion: We highlight the chemo-diversity of the antibacterial anthraquinones in the roots of Morinda morindoides.
Although tunas represent a significant part of the global fish economy and a major nutritional resource worldwide, their microbiome still remains poorly documented. Here, we conducted an analysis of the taxonomic composition of the bacterial communities inhabiting the gut, skin, and liver of two most consumed tropical tuna species (skipjack and yellowfin), from individuals caught in the Atlantic and Indian oceans. We hypothesized that each organ harbors a specific microbial assemblage whose composition might vary according to different biotic (sex, species) and/or abiotic (environmental) factors. Our results revealed that the composition of the tuna microbiome was totally independent of fish sex, regardless of the species and ocean considered. Instead, the main determinants of observed diversity were (i) tuna species for the gut and (ii) sampling site for the skin mucus layer and (iii) a combination of both parameters for the liver. Interestingly, 4.5% of all amplicon sequence variants (ASV) were shared by the three organs, highlighting the presence of a core-microbiota whose most abundant representatives belonged to the genera Mycoplasma, Cutibacterium, and Photobacterium. Our study also revealed the presence of a unique and diversified bacterial assemblage within the tuna liver, comprising a substantial proportion of potential histamine-producing bacteria, well known for their pathogenicity and their contribution to fish poisoning cases. These results indicate that this organ is an unexplored microbial niche whose role in the health of both the host and consumers remains to be elucidated.
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