Antioxidants are a family of substances that can neutralize free radicals and prevent and/or treat diseases associated with oxidative stress such as male’s infertility. Medicinal plants are one of the main sources of antioxidants. Aim: This work was aimed at evaluating the chemical and antioxidant potential of different extracts from some plants used in traditional Beninese medicine for the treatment of male infertility. Materials and Methods: The study was carried out on aqueous, hydro-ethanolic and ethanolic extracts from of the roots of Gardenia ternifolia (G. ternifolia), the whole plant of Cassytha filiformis (C. filiformis), the leaves of Rourea coccinea (R. coccinea) and the seed of Garcinia kola (G .kola). Quantification of the total polyphenols and flavonoids content of these extracts was evaluated respectively by the method using Folin Ciocalteu and the method using Aluminum trichloride. The antioxidant activity of the extracts was evaluated by molecular spectrophotometry using the free radical scavenging of DPPH and FRAP methods. Results: The results obtained indicated a variation of total polyphenols and flavonoids content according to the type of extract. Hydro-ethanolic extract of the various plants studied has a high polyphenols and flavonoids content. In variable proportions, all the extracts tested reduced the DPPH radical and ferric iron, reflecting their antioxidant potential. The best antioxidant activity has been obtained with the hydro-ethanolic extracts. Conclusion: This study showed that all the plant's extracts studied have antioxidant activity that varies with the type of extract. However, the hydro-ethanolic extractions showed the best antioxidant activities. The data obtained in the present study justified the use of these plants in management of pathologies involving oxidative stress.
Objective: Aristolochia albida Duch is frequently used by alternative medicine to treat some pathologies like hepatitis. Our main objective was to evaluate the acute oral toxicity of the ethanolic extract of this plant.Methods: Exploratory tests for acute oral toxicity are performed in vivo on Wistar albino rats in a limit test of 2000 mg/kg for 14 d in accordance with the OECD Guidelines 423. The clinical signs were observed every day, followed by measurement of body weight change, the haematological and biochemical examinations were executed and statistical analysis was performed. Results:The various clinical signs observed after administration and for 14 d were recorded and no mortality was observed. With the exception of white blood cells, mean cell volume and platelets with statistically significant difference in control (p<0.05), all the haematological parameters showed an insignificant statistical difference to the control ratio (p>0.05). For biochemical parameters, except blood glucose and total protein of control batches, which show a significant statistical difference after 14 d (p>0.05), all the biochemical parameters show the statistically insignificant difference for test and controls batches (p>0.05) as well as the weight variation of the animals. Conclusion:The ethanolic extract of the leaves of A. albida Duch (EEAr) had not toxic effect on the biochemical and hematological parameters studied at a dose of 2000 mg/kg. The lethal dose is therefore over 2000 mg/kg.
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