This study shows that the real time PCR test could be useful to identify HCMV DNA in saliva and to monitor patients at risk of cytomegalovirus disease after allogeneic hematopoietic stem cell transplant. However, further studies are necessary to confirm this data.

Background: The association between cell phone use and the development of parotid tumors is controversial. Because there is unequivocal evidence that the microenvironment is important for tumor formation, we investigated in the parotid glands whether cell phone use alters the expression of gene products related to cellular stress.Methods: We used the saliva produced by the parotid glands of 62 individuals to assess molecular alterations compatible with cellular stress, comparing the saliva from the gland exposed to cell phone radiation (ipsilateral) to the saliva from the opposite, unexposed parotid gland (contralateral) of each individual. We compared salivary flow, total protein concentration, p53, p21, reactive oxygen species (ROS), and salivary levels of glutathione (GSH), heat shock proteins 27 and 70, and IgA between the ipsilateral and contralateral parotids.Results: No difference was found for any of these parameters, even when grouping individuals by period of cell phone use in years or by monthly average calls in minutes.Conclusion and Impact: We provide molecular evidence that the exposure of parotid glands to cell phone use does not alter parotid salivary flow, protein concentration, or levels of proteins of genes that are directly or indirectly affected by heat-induced cellular stress. Cancer Epidemiol Biomarkers Prev; 23(7);

Decreased IL-1β and increased IL-10 levels in the blood are associated with lower survival. HCMV genotypes are associated with different cytokine levels in saliva and blood.

The aim of the study is to investigate if larger oral squamous cell carcinoma (OSCC) tumors show a different transcriptional signature of cell cycle genes in relation to small OSCC tumor samples. To address this issue, 17 fresh OSCC tumor samples were included in the study, including T1(n = 5),T2 (n = 9),T3 (n = 2) and T4 (n = 1) samples. All tumors were either from the tongue or from the floor of the mouth and only two patients were non-smokers. Two samples were from female patients, while the other 15 were from men. Samples were categorized according to clinical tumor size in tumors ≤ 2cm (T1 n = 5) or tumors > 2cm (T2, T3 and T4, n = 12). The median age of both groups was similar (57 and 62, respectively). The group of tumors ≤ 2cm was considered the reference group in the qRT-PCR results interpretation, while the larger tumors were considered the test group. The RNA was extracted and the cDNA was synthesized. A commercially available 84 cell cycle genes PCR array kit was used (PAHS-020, Cell Cycle PCR Array - Human, Qiagen). The results were analyzed according to the formula 2^-deltaCT and differential expressions were considered statistically significant when p<0.05. The target genes expressions were normalized to two housekeeping endogenous genes B2M and HPRT1, considering both, the geometric and arithmetic CT means (that differed less than 1 CT between the reference and test groups). The following genes were downregulated in the test group (larger tumors) compared to the reference group (smaller tumors), showing a minimum fold change of 4: ANAPC4, E2F4, CUL1, PCNA, KNTC1, RPL13A, KPNA2, NBN, CCNG2 and SUMO1. These results need to be further confirmed in a larger cohort of samples. In conclusion, these findings suggest that the transcriptional activity of specific cell cycle genes changes according to the size of the OSCC tumor, which probably reflects tumor molecular evolution.Supported by: FAPEMIG (Fundação de Amparo à Pesquisa de Minas Gerais), CNPq (National Council for Scientific and Technological Development) and CAPES (Coordination for Improvement of Higher Education Personnel), Brazil Note: This abstract was not presented at the meeting. Citation Format: Carolina C. Gomes, Marina G. Diniz, Fabricio T. de Souza, Jeane F. Correia-Silva, Ricardo S. Gomez. qPCR array of 84 cell cycle genes in oral squamous cell carcinoma reveals differently expressed genes in larger size tumors in relation to small tumors. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2206. doi:10.1158/1538-7445.AM2015-2206

The association between cell phone use and the development of parotid tumors is controversial. Because there is unequivocal evidence that the microenvironment is important for tumor formation, we investigated in the parotid glands whether cell phone use alters the expression of cytokines. We used the saliva produced by the parotid glands to assess cytokines levels, comparing the saliva from the gland exposed to cell phone radiation (ipsilateral) to the saliva from the opposite, unexposed parotid (contralateral) of each individual. We compared salivary flow, total protein, IL-1 β, IL-6, IFN-γ and TNF-α between the ipsilateral and contralateral parotids of forty-three individuals. No difference was found for any of these parameters. Our study provides evidence that the exposure of parotid glands to cell phone use does not alter cytokines levels in the parotid. Supported by CAPES, FAPEMIG and CNPq, Brazil. Citation Format: Fabrício TA de Souza, Carolina C. Gomes, Luiz Armando De Marco, Elisa C. Siqueira, Samuel M. Costa, Jeane F. Correia-Silva, Ricardo S. Gomez. Cell phone use and cytokines expression in saliva of the parotid glands. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3297. doi:10.1158/1538-7445.AM2015-3297