We conducted this study to determine the fatty acid composition of breastmilk of Israeli women and compare it with baby milk substitutes. Samples of mature breast milk, from 29 lactating Israeli Jewish mothers were collected during feedings. Total milk lipids extracts were transmethylated and analyzed by using an improved gas-chromatographic method. About 72% of the total fatty acids in the investigated breast milk comprised oleic (18:1c; 31±4%), palmitic (16:0; 21±4%), and linoleic (18:2n-6; 20±4%) acids. Total saturated fatty acids represented 42±7% of total fatty acids. The monounsaturated fatty acids content was 33±5%, of which 94% was oleic acid, and the polyunsaturated fatty acids content was about 24±4%, of which 85% was linoleic acid. The a-linolenic acid level found in this study, 2.0±0.6%, was higher than the range of values reported for the world population (0.10-1.4%). The main fatty acids composition of the milk substitutes has very similar composition to the breast milk. Docosahexaenoic acid levels are particularly low among the population of Jewish nursing mothers in relation to the milk substitutes, containing docosahexaenoic acid in their formulations.
Four commercial brands of infant milk formula were investigated, and the amount of shelf lipid peroxidation was determined by measuring malondialdehyde (MDA) or, more exactly, thiobarbituric acid reactive substance (TBARS) levels, which are the end products of lipid peroxidation. TBARS level, indicated by MDA concentration, was higher in the vegetarian brands. The vegetarian brands are, as expected, more prone to oxidation than dairy brands, as they contain more unsaturated fats. The introduction of formate anion diminishes the peroxide amounts initially present in the milk samples as well as those induced by radiation-induced oxidation.
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