Objective-High-frequency oscillations (HFOs) in the intracerebral electroencephalogram (EEG) have been linked to the seizure onset zone (SOZ). We investigated whether HFOs can delineate epileptogenic areas even outside the SOZ by correlating the resection of HFO-generating areas with surgical outcome.Methods-Twenty patients who underwent a surgical resection for medically intractable epilepsy were studied. All had presurgical intracerebral EEG (500Hz filter and 2,000Hz sampling rate), at least 12-month postsurgical follow-up, and a postsurgical magnetic resonance imaging (MRI). HFOs (ripples, 80 -250Hz; fast ripples, >250Hz) were identified visually during 5 to 10 minutes of slow-wave sleep. Rates and extent of HFOs and interictal spikes in resected versus nonresected areas, assessed on postsurgical MRIs, were compared with surgical outcome (Engel's classification). We also evaluated the predictive value of removing the SOZ in terms of surgical outcome.Results-The mean duration of follow-up was 22.7 months. Eight patients had good (Engel classes 1 and 2) and 12 poor (classes 3 and 4) surgical outcomes. Patients with a good outcome had a significantly larger proportion of HFO-generating areas removed than patients with a poor outcome. No such difference was seen for spike-generating regions or the SOZ.Interpretation-The correlation between removal of HFO-generating areas and good surgical outcome indicates that HFOs could be used as a marker of epileptogenicity and may be more accurate than spike-generating areas or the SOZ. In patients in whom the majority of HFOgenerating tissue remained, a poor surgical outcome occurred.Thirty percent to 40% of patients with focal epilepsy are medically intractable, 1 and for some, surgical removal of epileptogenic areas is the best option to gain seizure freedom. Intracranial electroencephalographic (iEEG) investigations are indicated for patients in whom noninvasive methods fail to identify a single focal seizure generator. CIHR Author Manuscript CIHR Author Manuscript CIHR Author ManuscriptiEEG is used to define the seizure onset zone (SOZ). 3 Removal of the SOZ alone, however, does not always predict the surgical benefit. 4,5 It is uncertain whether the outcome can be improved by removing areas of interictal spiking, often more widespread than the SOZ. 6,7 Intracranial studies also have limitations, as their results depend on electrode location and type of implantation (intracortical vs subdural). For instance, iEEG electrodes only record neuronal activity in their direct vicinity and are blind for other areas, 8 making it hard to judge whether the activity at seizure onset really represents the seizure generator or is the result of propagation from else-where. Thus the actual focus and its extent may be missed, leading to unsuccessful surgery.Microelectrode-recorded high-frequency oscillations (HFOs), ripples (80 -250Hz), and fast ripples (FRs, 250 -500Hz), were found predominantly in epileptogenic tissue. 9 -11 They can also be recorded with macroelectrodes duri...

Lampreys are representatives of an ancient vertebrate lineage that diverged from our own ~500 million years ago. By virtue of this deeply shared ancestry, the sea lamprey (P. marinus) genome is uniquely poised to provide insight into the ancestry of vertebrate genomes and the underlying principles of vertebrate biology. Here, we present the first lamprey whole-genome sequence and assembly. We note challenges faced owing to its high content of repetitive elements and GC bases, as well as the absence of broad-scale sequence information from closely related species. Analyses of the assembly indicate that two whole-genome duplications likely occurred before the divergence of ancestral lamprey and gnathostome lineages. Moreover, the results help define key evolutionary events within vertebrate lineages, including the origin of myelin-associated proteins and the development of appendages. The lamprey genome provides an important resource for reconstructing vertebrate origins and the evolutionary events that have shaped the genomes of extant organisms.

Friendship expectations are prescriptive normative behaviors and highly valued qualities in ideal same-sex friends. This paper reports the results of five meta-analyses of sex differences from 37 manuscripts (36 samples, N = 8825). A small difference favoring females was detected in overall friendship expectations ( d = .17). Friendship expectations were higher for females in three of four categories: symmetrical reciprocity (e.g., loyalty, genuineness; d = .17), communion (e.g., self-disclosure, intimacy; d = .39), solidarity (e.g., mutual activities, companionship; d = .03), but agency (e.g., physical fitness, status; d = -.34) was higher in males. Overall expectations and symmetrical reciprocity showed small effect sizes. Medium effect sizes for communion favoring females and for agency favoring males support predictions of evolutionary theory.

This article uses dialectical theory to examine how mobile phone use in close friendships affects relational expectations, the experiences of dependence, overdependence, and entrapment, and how those experiences affect relational satisfaction. Results suggest that increased mobile phone use for the purpose of relational maintenance has contradictory consequences for close friendships. Using mobile phones in close relationships increased expectations of relationship maintenance through mobile phones. Increased mobile maintenance expectations positively predicted dependence, which increased satisfaction, and positively predicted overdependence, which decreased satisfaction. Additionally, entrapment, the guilt and pressure to respond to mobile phone contact, uniquely predicted dissatisfaction. The results are interpreted in relation to the interdependent dialectical tensions of friendship, media entrapment, and the logic of perpetual contact.