Juvenile silky sharks Carcharhinus falciformis comprise the largest component of the incidental elasmobranch catch taken in tropical tuna purse seine fisheries. During a chartered cruise on board a tuna purse seine vessel conducting typical fishing operations we investigated the post-release survival and rates of interaction with fishing gear of incidentally captured silky sharks using a combination of satellite linked pop-up tags and blood chemistry analysis. To identify trends in survival probability and the point in the fishing interaction when sharks sustain the injuries that lead to mortality, sharks were sampled during every stage of the fishing procedure. The total mortality rates of silky sharks captured in purse seine gear was found to exceed 84%. We found survival to precipitously decline once the silky sharks had been confined in the sack portion of the net just prior to loading. Additionally, shark interactions recorded by the scientists were markedly higher than those recorded by vessel officers and the fishery observer. Future efforts to reduce the impact of purse seine fishing on silky shark populations should be focused on avoidance or releasing sharks while they are still free swimming.