Biocompatible 7-methoxy coumarin encapsulated 7-MC@ZIF-L nanocomposite was fabricated to develop efficient wound healing. The stimuli-responsive drug release mechanism was examined at pH 5 and 7.4. The release of coumarin was increased within three hours by burst release under acidic conditions. The 7-MC@ZIF-L nanocomposite shows significant bacterial growth control against human infectious pathogens S. aureus, E. coli, P. aeruginosa, and S. epidermis. Moreover, the MTT and AO/EB assays revealed that nanocomposite had high cell viability towards L929 fibroblast cells. The present study enlightens a novel one-pot fabrication of 7-MC@ZIF-L nanocomposite as a drug delivery system and its stimuli-responsive drug release ability. The current methodology is an effective approach than any existing post-synthesis methods. The synthesized 7-MC@ZIF-L nanocomposite demonstrates ultrahigh thermal and chemical stability. The anti-biofilm results confirmed that the nanocomposite effectively inhibits biofilm formation and damages the biofilm architectural matrix based on the dose-dependent manner. In vitro wound scratch assay revealed that the sustainable and slow release of 7-methoxy coumarin and zinc ions promotes the cell proliferation rate. Bio-compatibility study results confirmed that the 7-MC@ZIF-L nanocomposite is safer and non-toxic for human use. In vivo artemia, salina lethality assay concluded that the nanocomposite is highly suitable for wound healing and other biomedical applications. The overall results signify that the 7-MC-ZIF-L nanocomposite is an efficient material for stimuli-responsive wound healing treatment in the future for wound care therapy.