A gene homologous to the IgD heavy chain (delta) gene in channel catfish (Ictalurus punctatus) was found 0.9 kb downstream of the IgM heavy chain (mu) gene in Atlantic salmon (Salmo salar). As in catfish, the first constant mu exon is spliced into the delta transcripts. In agreement with the tetraploid ancestry of the salmonid fish family there are two highly similar delta genes in Atlantic salmon. Characterization of these genes showed that they encode seven 'unique' Ig domains, three of which are tandem duplicated, i.e. like delta1-(delta2-delta3-delta4)*-(delta2- delta3-delta4)-delta5-delta6-d elta7. Sequence analysis indicates that delta1-delta7 arose from two duplication events. Accordingly, salmon delta can be reduced to a unit of three Ig domains corresponding to the three C-terminal domains of a prototypic Ig molecule. The ancestral three-domain unit is apparently best conserved in delta1-delta5-delta6. Phylograms indicate a relationship between teleost and mammalian IgD mainly because of the similarity between the teleost delta5 and human delta2. The corresponding domain in mouse IgD has been deleted during evolution. The teleost delta1 and delta6 sequences are most similar to domains of other non-IgM isotypes, including those in cartilaginous fishes.

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In this study we present the cloning, expression and partial identification of Genomic Segment 7 of infectious salmon anaemia virus (ISAV). The nucleotide sequence corresponding to Segment 7 was isolated from a bacteriophage lambda cDNA library and contained 2 overlapping open reading frames (ORFs) of 903 and 522 bases respectively. It also contained an ISAV-specific conserved nucleotide motif in the mRNA 5' region. The co-linear transcript representing the large ORF undergoes a splicing event that removes a 526 nucleotide intron to form a mRNA corresponding to the smaller reading frame. Thus, ISAV Genomic Segment 7 has a similar coding strategy as influenza A virus Segments 7 and 8. The largest ORF of Segment 7 and the first ORF of Segment 8 was expressed in E. coli as fusion proteins and rabbit antiserum was raised against the recombinant protein from Segment 8. Immunoblot studies using this antiserum and a serum against purified virus, show that Segment 8 encodes one of the major structural proteins of the virus whereas the co-linear ORF of Segment 7 probably encodes a non- or minor structural protein

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