No abstract

Despite being described as the safest antiepileptic drug of first choice the presented literature data are much varied as far as dog blood serum biochemical parameters are considered. The aim of this study was to investigate the effect of phenobarbitone at different per os doses on the values of selected blood serum biochemical parameters in dogs during both short and long term application. The study was conducted on 30 dogs of different races, both sexes, ranging from 2 to 8 years of age. A total of 15 healthy and 15 dogs suffering from idiophatic epilepsy were observed. During the short term per os application of phenobarbitone (given at 3 week intervals) to the healty population in varied doseses a statistically significant increase in ALT and AP was recorded. Application of 16 mg/kg/day of phenobarbitone to the healthy population during 14 days resulted in a significant increase of ALT ans AP. This increase was dependant on the duration of the treatment. During chronic application of phenobarbitone to dogs suffering from idiopathic epilepsy a significant increase in values of AP and ALT depending on the given dose was recorded. In two of the studied epileptic dogs treated with high therapeutic doses of phenobarbitone clinical signs of hepatotoxicity were recorded. Hepatotoxicity resulted in decreased albumin and total protein concentrations, as well as increased blood serum total bilirubin, AST, ALP and AP. The obtained results indicate that a long term application of phenobarbitone at high therapeutic doses can cause hepatotoxicity. However, there was no relationship between phenobarbitone dosage and duration of therapy and blood glucose, urea, creatinine, total proteins, albumins, total bilirubin, triglycerides and cholesterol

Anesthetic agents could alter the course and outcome of physical trauma, as well as experimentally or naturally occurring severe infections, by regulating several immune response mechanisms. The aim of our study was to investigate the influence of several commercially used anesthetic agents (ketamine, propofole, pentylentetrazole - PTZ) on cytokine concentrations, animal survival and pathohistological changes in the model of rat sepsis. In adult, male Wistar rats after different anesthetic treatment and induction of sepsis by cecal ligation and punction we estimated serum levels of IL1α, TNFα, GM-CSF and MCP-1 at 12h intervals. After 48h of sepsis induction, the largest number of animals survived in the group treated with PTZ (47%), while the lowest survival rate was in the propofole treatment group (24%). Contrary to survival rate, the most abundant pathohistological changes were seen on preparations from PTZ and than in ketamine/PTZ treated groups, without any significant changes in the CNS of propofole treated animals. In the propofole treated group there was a prominent increament of GM-CSF values at 12h and 24h, followed by a significant decreament at 36h. These changes were negatively correlated to the survival rate in this group. This group had the lowest levels of MCP1 at all evaluated time intervals. After high initial levels, IL1α and TNFα levels fell to undetectable concentrations and at 24h increased to a high level. In PTZ as well as ketamine groups, at 12 h interval, GM-CSF levels were lower than in the propofole treated group. Contrary, MCP-1 levels were higher in these groups comparing to propofole group. After a high initial peak, IL1α levels decreased to low but detectable levels, followed by an intensive rise in ketamine treated, but with further decrement in pentazole treated groups. TNFα levels were low through all evaluated intervals in both these groups. Our results indicate that induction of anaesthesia of animals with sepsis with variuos anesthetic agents is connected to different pathohistological CNS changes, distinct serum cytokine profiles and diverse survival rates

Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix char-

Histological evaluations of soft tissue reactions in implanted biomaterials is a long established method of investigating their biocompatibility. It is a very important procedure convenient for various biomaterials, as appropriate for soft as for hard tissue, but at the same time this method is not sufficient for a prediction of their clinic performance. The wide spread practice of this method is in the simplicity of its use in subcutaneous or intramuscular implantations on the animal model, and consequently exploring principal and surrounding tissue is very simple with screening tests for esimating the irritaton index and interaction between tissue and biomaterials. The screening tests (evaluation tests) involve implantation of biomaterials in various locations in the body, different tissue treatment as immunohistochemical tissue analysis, transmissional, scanning and confocal microscopy. Interactions among soft tissue with bioceramics and the evaluation of their cellular and tissue performance in inflammation and immunological response are the first step in the estimation of biocompatibility. [Projekat Ministarstva nauke Republike Srbije, br. 175061