Couples’ HIV Counseling and Testing (CHCT) is an essential component of an effective response to AIDS pandemic worldwide and serves as an entry point to HIV care and support. However, it has become increasingly apparent that couples have overlooked the services yet they are too at risk of HIV infection. This study sought to determine level of acceptance of CHCT among pregnant women attending antenatal care services in Likoni sub-County, Mombasa County, Kenya. A descriptive cross-sectional study design was used with structured questionnaires and an observation checklist to collect data from couples while an interview schedule was used to solicit data from health care workers. Results showed uptake of CHCT in the study area was relatively low (33.5%). Statistically significant associations were found between CHCT and demographic factors such as education (χ2 = 29.54; p = 0.005), marital status (χ2 = 12.41; p = 0.010), occupation (χ2 = 18.18; p < 0.001), income (χ2 = 23.02; p < 0.001). In operation factors, statistically significant associations were found between CHCT and distance to health facility (χ2 = 8.31; p = 0.040), means of transport (χ2 = 10.28, p = 0.040), cost of transport (χ2 = 10.92; p = 0.010), appropriateness and convenience of time allocated (χ2 = 11.88, p <, 0.001), an individual’s working hours per day (χ2 = 14.46; p < 0.001), and education/information on CHCT (χ2 = 5.97; p = 0.020). In HIV related knowledge, attitude and practice of couples on HIV risk behavior, significant associations were found between CHCT and prevention of discordant partners from contracting HIV (χ2 = 53.71; p < 0.001), helping couples adhere to ART (χ2 = 43.34; p  < 0.001), helping to reduce morbidity and mortality due to HIV and other opportunistic infections (χ2 = 48.66; p < 0.001), increasing trust among partners (χ2 = 45.16; p < 0.001), and increasing marital cohesion among partners (χ2 = 65.78; p < 0.001). Logistic regression revealed that determinants of CHCT were significant for married status (p < 0.001), unemployed (p = 0.020), that CHCT increases trust among partners (p = 0.020), and that CHCT increases marital cohesion among partners (p = 0.001). Based on these findings, there is need to improve awareness on CHCT to enhance positive attitudes towards couples testing.