Healthcare-associated infections are associated with significant increased cost of healthcare services, days of hospitalisation and mortality. 9 Healthcare-associated infections caused by multidrugresistant bacteria phenotypes, such as extended spectrum β-lactamase-producing GNB (ESBL-GNB), further exaggerate morbidity and mortality. At the study site in Mwanza, Tanzania, the prevalence of HCAIs in surgical site infections ranges from 10% to 26%. 5,9,10 Staphylococcus aureus accounts for nearly one-third of these, of which about 16% to 19% are methicillin resistant. 5,9 On the other hand, only one study reported 13% of implicating GNB showed ESBL phenotypes. 9 To date, the distribution of ESBL genes among ESBL-GNB phenotypes causing HCAIs is not clearly known. This study unravels the distributions of ESBL genes (bla CTX-M , bla TEM and bla SHV ) among ceftriaxoneresistant GNB causing HCAIs at a zonal referral hospital in Mwanza, Tanzania.