Fine needle puncture (FNP) is a widespread technique used to collect cellular samples. Its efficiency can be enhanced by the use of ultrasonography to guide the procedure. Ultrasound-guided fine needle puncture is therefore an operator-dependent exam. For this reason, it demands the acquisition of psychomotor skill, ability to recognize structures and dexterity during the needle puncture. This study described the development of an artisanal simulator made with gelatin to replace the use of live animals during practical classes in Veterinary or Medical Sciences education. The experimental set consisted of three phases in which the student should perform different tasks such as recognition of the target structure with the ultrasound and injection of ink (phase 1) or aspiration (phase 2) of its content; and evaluation of a parenchymatous organ (liver) and puncture of its surface (phase 3). A survey on the acceptance of the model was carried out and students filled a questionnaire elaborated with the Visual Analog Scale system. Participants considered the artisanal model as a strong method to teach ultrasound-guided FNP. Other attractive advantages of this simulator are the low manufacturing costs (compared to expensive high-technology devices) and the possibility to replace the use of live animals in practical classes.