Losses in seed yield and quality caused by infection with Beet western yellows virus (BWYV) alone or in combination with direct feeding damage by Myzus persicae (green peach aphid) were quantified in field experiments with Brassica napus (canola, oilseed rape) in the ‘grainbelt’ region of south-western Australia. Plants infected with BWYV and infested with M. persicae were introduced into plots early to provide infection sources and spread BWYV to B. napus plants. Insecticides were applied as seed dressings and/or foliar applications to generate a wide range of BWYV incidences in plots. Colonisation by vector aphids and spread of BWYV infection were recorded in the plots of the different treatments. At sites A (Medina) and B (Badgingarra) in 2001, foliar insecticide applications were applied differentially at first, but, later, ‘blanket’ insecticide sprays were applied to all plots to exclude any direct feeding damage by aphids. When BWYV infection at sites A and B reached 96% and 100% of plants, it decreased seed yield by up to 46% and 37%, respectively. Also, variation in BWYV incidence explained 95% (site A) and 96% (site B) of the variation in yield gaps, where for each 1% increase in virus incidence there was a yield decrease of 12 (site A) and 6 (site B) kg/ha. At both sites, this yield decline was entirely because fewer seeds formed on infected plants. At site B, BWYV infection significantly diminished oil content of seeds (up to 3%), but significantly increased individual seed weight (up to 11%) and erucic acid content (up to 44%); significant increases in seed protein content (up to 6–11%) were recorded at both sites. In field experiments at sites B and C (Avondale) in 2002, insecticides were applied as seed dressings or foliar sprays. At site B, when BWYV incidence reached 98%, the overall yield loss caused by BWYV and direct M. persicae feeding damage combined was 50%. At site C, when BWYV incidence reached 97%, the overall combined yield decline caused by BWYV and direct feeding damage was 46%. This research under Australian conditions shows that, when aphids spread it to B. napus plantings such that many plants become infected at an early growth stage, BWYV has substantial yield-limiting potential in B. napus crops. Although the results represent a worst case scenario, the losses were greater than those reported previously in Europe and are cause for concern for the Australian B. napus industry. When applied at 525 g a.i./100 kg of seed, imidacloprid seed dressing controlled insecticide-resistant M. persicae and effectively suppressed spread of BWYV for 2.5 months and increased seed yield by 84% at site B and 88% at site C. Therefore, provided that mixing the insecticide with seed is sufficiently thorough, dressing seed with imidacloprid before sowing provides good prospects for control of BWYV and M. persicae in B. napus crops.

During the summer periods of 2000 and 2001, incidences of infection with Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV) were determined in grass weeds and volunteer cereals surviving at isolated sites throughout the grainbelt of south-western Australia, which has a Mediterranean-type climate. Samples of Cynodon dactylon, Eragrostis curvula, Erharta calycina, Pennisetum clandestinum, and volunteer cereals (mostly wheat) were tested for BYDV (serotypes MAV, PAV and RMV) and CYDV (serotype RPV), and those of at least 19 other grass species were tested for BYDV only (serotypes PAV and MAV). In 2000, BYDV and/or CYDV were detected in 33% of 192 sites in 0.7% of 26 700 samples, and in 2001 the corresponding values were 19% of 176 sites and 0.5% of 21 953 samples. Infection was distributed relatively evenly throughout the different annual average rainfall zones of the grainbelt, but when sites were categorised according to actual rainfall for late spring to early autumn, the proportion of sites and samples infected increased where such rainfall exceeded 300 mm. In both summer sampling periods, the most abundant grass species were C. dactylon and E. curvula, with BYDV and/or CYDV being detected in 0.1–0.6% and 0.1–0.5% of samples, respectively. The corresponding incidences were 0–1% for Erharta calycina, 7–8% for P. clandestinum, and 0.2–2% for volunteer wheat. The most abundant species tested for BYDV only were Chloris truncata and Digitaria sanguinalis, with infection incidences of 0.2–0.7 and 0.2–0.3%, respectively. Chloris virgata (2–3%) and Urochloa panicoides (0.3–0.6%) were the only other infected species. Within individual sites and host species, the greatest incidences of CYDV were in P. clandestinum (23% in 2000 and 18% in 2001) and of BYDV in Chloris virgata (14% with PAV and 12% with MAV in 2000). Small populations of grass-infesting aphids were found over-summering at 26% (2000) and 3% (2001) of sites and occurred in all 3 annual rainfall zones. The predominant species was Hysteroneura setariae, but Rhopalosiphum maidis, R. padi, and Sitobion miscanthi occurred occasionally. Presence of over-summering BYDV, CYDV, and aphids in all rainfall zones has important implications for virus spread to cereal crops throughout the grainbelt.

During the summer periods of 2000, 2001, and 2002, presence of Beet western yellows virus (BWYV) was assessed in tests on samples from at least 12 broad-leafed weed species and 5 types of volunteer crop plants growing in the grainbelt region of south-western Australia. In 2000, BWYV was detected in 2 of 35 sites in 2% of 1437 samples, whereas in 2001 and 2002 the corresponding figures were 3 of 108 sites in 0.04% of 8782 samples, and 1 of 30 sites in 0.08% of 2524 samples, respectively. The sites with infection were in northern, central, and southern grainbelt districts, and in high and medium rainfall zones. The hosts in which BWYV was detected were the weeds Citrullus lanatus (Afghan or wild melon), Conzya spp. (fleabane), Navarretia squarrosa (stinkweed), and Solanum nigrum (blackberry nightshade), and the volunteer crop plant Brassica napus (canola). Small populations of aphids were found over-summering at 28% (2000), 4% (2001), and 17% (2002) of sites, mostly infesting volunteer canola and Raphanus raphanistrum (wild radish). They occurred in high, medium, and low rainfall zones, but were only found in central and southern grainbelt districts. The predominant aphid species found was Brevicoryne brassicae, with Acyrthosiphon pisum, Brachycaudus helichrysi, Hyperomyzus lactucae, Lipaphis erysimi, Myzus persicae, and Uroleucon sonchi present occasionally. The importance of these findings in relation to the epidemiology and control of BWYV in the grainbelt is discussed.

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