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In this study, we investigated the interaction of U­(VI) and Eu­(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0–200 μM) bioassociation experiments showed that more than 75% of U­(VI) and Eu­(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multistage bioassociation process for cells that were exposed to 200 μM U­(VI), where, after initial immobilization of U­(VI) within 1 h of exposure, it was released back into the culture medium starting within 24 h. A remobilization to this extent has not been previously observed. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the remobilization of U back into the solution, which we assume to be a U­(VI) phosphate species. Thus, the interaction of U­(VI) and Eu­(III) with released plant metabolites could be concluded.

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