The proteolytic systems of lactic acid bacteria are important as a means of making protein and peptide N available for growth and as part of the curing or maturation processes which give foods their characteristic rheological and organoleptic properties. The proteolytic systems of lactic acid bacteria are described in relation to their growth and their functions in protein-rich foods. Their role in the manufacture of milk products is discussed.
Solubilized cell walls of group N streptococci contain two electrophoretically distinct peptidases, one of which hydrolysed trileucine only, while the second hydrolysed a wide range of di- and tripeptides. Neither enzyme possessed leucine aminopeptidase or endopeptidase activity. Four and three peptidases, respectively, were separated in intracellular extracts of Streptococcus lactis subsp. lactis and Strep. lactis subsp. cremoris produced by osmotic lysis of spheroplasts. In contrast with the cell-wall extracts, two of the peptidases had broad specificites, though only one of these hydrolysed trileucine. Purified membranes of Strep. lactis subsp. lactis contained only one electrophoretically distinct peptidase of very narrow specificity. There were small differences between the numbers of peptides hydrolysed by cell wall preparations from milk-grown or broth-grown cells.
In aseptic filling systems, hydrogen peroxide vapour is commonly used for the reduction of microbial contaminations in carton packages. In this process, the germicidal efficiency of the vapour depends especially on the H2O2 concentration. To monitor the H2O2 concentration, a calorimetric H2O2 gas sensor based on a catalytically activated thin‐film thermopile is investigated. Two different sensor layouts, namely a circular and a linear form, as well as two various material pairs such as tungsten/nickel and gold/nickel, have been examined for the realization of a thin‐film thermopile. Additionally, manganese oxide and palladium particles have been compared as responsive catalysts towards H2O2. The thin‐film sensors have been investigated at various H2O2 concentrations, gas temperatures and flow rates.
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