Introduction: The human face is a disconcerting source of information, since cultural influences, masculine-feminine characteristics, the aging process, the subtleties of light that change with the orientation of the face, rest, smiling and others configurations endow each particular face with a uniqueness that is difficult to quantify. The objective of this study was to determine whether or not there is a golden ratio between the intercanine distance and the vertical measurement of the midface of fourth-year students of the Faculty of Dentistry of the University of Cuenca. Methods: Descriptive, cross-sectional and randomized study. The sample consisted of 40 fourth-year students from the Faculty of Dentistry of the University of Cuenca, Ecuador. The observation technique was used. The following were observed and analyzed: 1) the intercanine distance and 2) the vertical measurement of the middle third of the face, to determine if there is a presence or absence of the golden ratio. Results: For the frequency of values about whether there is a Golden Ratio or not, it is shown that: in the intervals 2.65 - 12.90 mm (very close) there are 4 men with 13.33 % and 3 women with 6 00 %, in the interval 12.91 - 23.16 mm (close) there are 8 men with 26.66 % and 15 women with 30.00 %, and in the interval 23.27 - 33.52 mm (distant) there are 3 men with 10.00 % and 7 women with 14.00 %. Conclusion: It is observed that in 56.66 % of the fourth-year students of the Faculty of Dentistry of the University of Cuenca there is a close correlation to the golden ratio between the intercanine distance and the middle third of the face, this being the most representative of the three intervals analyzed (very close, close and distant).