Purpose: The purpose of this study was to investigate the potential of Persicaria thunbergii (P. thunbergii) extracts as physiologically active cosmetic ingredients. Methods: To elucidate the anti-oxidative, anti-inflammatory, and anti-microbial properties of P. thunbergii , its ethanol and hot-water extracts were examined for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide (NO) production, protective effects against oxidative stress in HaCaT cells, antiinflammatory activity, anti-microbial activity, and inhibition of β-hexosaminidase expression, an allergy factor. Results: The anti-oxidative activity of the two P. thunbergii extracts was compared, and the anti-oxidative activity of the ethanol extract was found to be superior to that of the hot-water extract. The polyphenol contents of P. thunbergii ethanol and hot-water extracts were 132.00 and 89.46 μg/ mL, respectively. And total flavonoid contents of P. thunbergii ethanol and hot-water extracts were 115.27, 84.94 μg/mL, respectively. The DPPH radical scavenging activity was found to be 79.80% for the ethanol extract and 70.33% for the hot-water extract at a concentration of 500 μg/mL. No significant cytotoxicity was observed in HaCaT, RAW 264.7 and RBL-2H3 cells. The protective effect of the extracts on HaCaT cell against oxidative stress induced by hydrogen peroxide (H 2 O 2) was confirmed by 23% for ethanol extract and 18% for hot water extract. The anti-inflammatory activity of the extracts was examined in RAW 264.7 cells, and nitric oxide (NO) production was suppressed in a concentration-dependent manner. Both ethanol and hotwater extracts also inhibited the degranulation of immune cells in a concentrationdependent manner, as assessed by the secretion of β-hexosaminidase. In addition, the concentration dependent anti-microbial activities of the extracts were demonstrated in several bacterial strains, such as those of Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and Propionibacterium acnes (P. acnes). Conclusion: Based on the findings from this study, P. thunbergii extracts could be used as functional cosmetic ingredients that possess anti-oxidative, antiinflammatory, and anti-microbial properties. In order to determine their applicability, further research should be systematically conducted to verify their efficacy through clinical studies in animals and humans.