Objective: Heart rate asymmetry is a phenomenon in which the contribution of heart rate decelerations to short-term heart rate variability is greater than that of accelerations, and the contribution of accelerations to long-term and total variability is greater than that of decelerations. This has been established for short, stationary recordings, so our aim is to do it for long recordings. Approach: In this paper, we analyze heart rate asymmetry in 87 long, 24 h electrocardiogram Holter recordings from healthy people. We show that in the whole recording all types of asymmetry are observable, clear and highly statistically significant. To analyze the local changes of asymmetry in time, we analyzed the recordings by disjoint jumping windows of 300 beats. Main results: This analysis revealed that the local, averaged measures of all types of asymmetry also demonstrate its presence which is highly statistically significant. Additionally, we introduce in this paper a statistical test for asymmetry in a single long recording, as opposed to the current approach in which whole groups are tested. We do this by introducing the proportion of time spent in asymmetry for each recording and using it in the binomial tests. Significance: We found that for all the recordings most of the time is spent in asymmetry.

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