The present knowledge concerning the glycan structures and role of glycoconjugates derived from amniotic fluid is fragmentary and mainly focuses on the individual glycoproteins. The question has arisen as whether the general glycosylation pattern of amniotic fluid glycoconjugates can change with the progression of a normal pregnancy. In the present work we have described the dynamic, quantitative alterations in relative amounts of sialic acid and fucose linked by a variety of anomeric linkages to subterminal oligosaccharide structures of amniotic fluid glycoconjugates in relation to pregnancy age. The analysis was performed in the following groups of amniotic fluids derived from normal pregnancy by lectin dotting method: "2nd trimester" (14-19 weeks), "3rd trimester" (29-37 weeks), "perinatal period" (38-40 weeks) , "delivery at term" (39-41 weeks) and "post date pregnancy" (41-43 weeks). In the "3rd trimester" the amniotic fluid glycoconjugates contained higher relative amounts of glycans terminated by alpha2-6-linked sialic acid (p < 0.00002) and by alpha1-6 innermost fucose (p < 0.000001) than those in the 2nd trimester. In contrast, they showed the lower relative amount of fucose linked alpha1-3 (p < 0.02). At the perinatal period the relative amount of alpha2-6-linked sialic acid increased (p < 0.03), and it then decreased during delivery (p < 0.02) to the level found in the "3rd trimester" group. In the post date pregnancy all parameters studied increased. The sialyl- and fucosyl-glycotopes of the amniotic fluid glycoconjugates may play an critical role in growth and tissue remodeling of the foetus, as well as may might reflect maturation of a foetus. Additionally, a determination of the glycotope expressions might be helpful in prenatal diagnosis as predictor factors for well being of mother and child.

Predisposing factors for the occurrence of a low rate of SOC in pregnant women are: lower age, multiparity, lack of social support, especially from the husband/partner, and the risk of depression during pregnancy. This may result from the fact that a strong SOC develops in the process of socialization, and with age we acquire the ability to accurately assess reality.

Introduction. Family planning methods let one make an informed decision about having children at the right time. People have been trying to adopt the right family planning methods for centuries, mainly through using various contraceptives. These used to be very diverse over the time, yet the goal remained the same – preventing pregnancy. Aim. Assessment of the factors affecting the knowledge and prevalence of contraceptive methods, as well as the factors affecting sexual behavior of adolescents/young adults. Material and methods. 219 individuals (111 women and 108 men) between 17-21 years of age, attending secondary schools, participated in this voluntary and anonymous study. A survey questionnaire prepared by the authors was used as a study tool. Statistical analysis of the data obtained was performed using the SPSS 17.0 software. Results. 81% of the adolescents/young adults stated that their knowledge of contraception is sufficient for them to be in charge of their own sexual lives, whilst some 9% mentioned that they lacked such knowledge, 85% of the young people studied stated that they were interested in contraception. The main sources of knowledge about contraception are the Internet (35%) and peers (22%). The survey showed that 59% of the individuals correctly answered the question regarding the length of a woman’s menstrual cycle, 77% correctly defined the first day of the cycle, and 49% correctly determined the time of ovulation. The studied group of people had difficulties determining menstrual cycle phases; 23% considered them as fixed, 27% – that they are variable, and 29% gave the correct answer. The surveyed people thought that contraceptive method effectiveness (43%), availability (33%) and ease of administration (17%) are most important when choosing the method. Condoms are the most common (72%) contraceptive method among the surveyed people while hormonal contraceptives and interrupted intercourse are poorly known (12% and 11%, respectively). The respondents deemed the hormonal method (50%), and intrauterine contraceptive methods (21%) and condoms (21%) the most effective modes of contraception. They thought that interrupted intercourse (53%) and natural birth control methods (34%) are the least effective. The age at which most of the respondents started their sexual life ranged between 16 and 18; with the age of 16 being the most common (24.2%). Some 40% of the surveyed called their sexual intercourse occasional. Conclusions. Adolescents consider both the Internet and their peers the primary source of information about sexuality, family planning or birth control methods. The average age of the first sexual experiences has been gradually decreasing and the condom remains to be the most common method of contraception for adolescents/young adults. The knowledge concerning family is insufficient and frequently inaccurate. Education in the fields of sexuality, contraceptive and family planning methods should be included in primary school curriculum.

The presented study aimed to evaluate in vitro the effectiveness of improvement standard chemotherapy with bleomycin by electroporation in two various ovarian cancer cell lines. Two human ovarian cell lines OvBH-1 and SKOV-3 were used. The lines were selected because of their resistance to several therapeutic methods. As anticancer drug we use range of concentrations of bleomycin. In EP and ECT experiments different voltage values: from 0 to 1200 V/cm, 8 pulses with duration of 100μs and intervals between pulses 1s long were used. The cells viability after applied treatments was evaluated by MTT assay. The expression of heat shock proteins - HSP27 was examined by immunocytochemical ABC method.The cytotoxicity with different concentrations of bleomycin alone was not significantly decrease in both cell lines. It confirms resistance of these cells to conventional chemotherapy. The highest decrease of cell proliferation was observed after EP with bleomycin after 48h of incubation for 1000 V/cm. The intensity of expression of small heat shock proteins HSP27 slightly increased after ECT in both treated cell lines, in particular in OvBH-1. The presented study indicated that application of electroporation may effectively enhance chemotherapy with bleomycin, particularly in the case of treating ovarian cancer resistant to standard therapy.

Betulinic acid (BTA) is naturally occurring triterpene that has received interest as a novel therapeutic substance with cytotoxicity towards a number of cancer cell lines. Despite the wide spectrum of biological and pharmacological effects, its effect may be limited its lipophobic properties. Therefore, strategies to improve the access of BTA to the cells are required to enhance the anticancer effects. Electroporation (EP) enables increased inflow of drugs into cancer cells, even at low doses, which may reduce the side effects caused by high doses of chemotherapy. The potential application of BTA in electrochemotherapy (ECT) in metastatic type of cancers was investigated in the present study. The efficacy of BTA with EP was estimated using a cell survival assay (MTT assay), microscopical morphology analysis and the immunocytochemical expression of heat shock proteins (HSPs). HSPs are molecules that protect the cell from harmful environmental, chemical and physical stresses, and ensure cell survival, recovery and proper functioning. HSP expression is induced various stress factors. Therefore, the expression of HSP27 and HSP70 was evaluated after cells were exposed to an external pulsed electric field and anticancer drugs. Facilitated drug delivery and the anticancer effect on metastatic tumor cells were evaluated in vitro. The effect of BTA was compared with cisplatin (CP), a standard cytostatic agent. Two different metastatic cancer cell lines were used, an ovary adenocarcinoma cell line (SW626) and melanoma cell line (Me45). BTA combined with EP exhibited similar efficacy to CP with EP after 24 and 48 h in SW626 and Me45 cancer cells. Me45 cells also had high HSP27 and low HSP70 immunosignals post-ECT treatment. ECT caused increased expression of HSP27 and HSP70 proteins in SW626 cells, which were less sensitive to ECT than the Me45 melanoma cell line. The results indicate that BTA may be efficiently applied instead of CP in ECT approaches, but its activity differs between tumor cell lines.