The aim of this study was to determine the suitability of the comparative genomic hybridization to microarray (aCGH) technique for prenatal diagnosis, but also to assess the frequency of chromosomal aberrations that may lead to fetal malformations but are not included in the diagnostic report. We present the results of the aCGH in a cohort of 7400 prenatal cases, indicated for invasive testing due to ultrasound abnormalities, high-risk for serum screening, thickened nuchal translucency, family history of genetic abnormalities or congenital abnormalities, and advanced maternal age (AMA). The overall chromosomal aberration detection rate was 27.2% (2010/7400), including 71.2% (1431/2010) of numerical aberrations and 28.8% (579/2010) of structural aberrations. Additionally, the detection rate of clinically significant copy number variants (CNVs) was 6.8% (505/7400) and 0.7% (57/7400) for variants of unknown clinical significance. The detection rate of clinically significant submicroscopic CNVs was 7.9% (334/4204) for fetuses with structural anomalies, 5.4% (18/336) in AMA, 3.1% (22/713) in the group of abnormal serum screening and 6.1% (131/2147) in other indications. Using the aCGH method, it was possible to assess the frequency of pathogenic chromosomal aberrations, of likely pathogenic and of uncertain clinical significance, in the groups of cases with different indications for an invasive test.

This article presents a new method of cranioplasty in which polypropylene polyester knitwear was used as the filling material. The basis for prosthesis shaping was a three-dimensional model of the defect made according to the patient's CT scans. Previously, such material has never been a subject of computer-aided design and computer-aided manufacturing (CAD/CAM) individual forming. The process of the prosthesis design included CT bone scans and mold preparation for each patient. Such prostheses were implanted in 48 patients with cranial defects. The total number of prostheses applied was 51. The follow-up time was at least 6 months up to 36 months. The group of treated patients is described here, and sample pictures are shown to illustrate the results. The smallest defect had a size of 15 cm(2); the biggest, 178 cm(2). The coverage and the aesthetic results were very good in all cases. Two patients had postoperative complications. The cranioplastic solution described here is a valuable addition to the existing reconstructive methods, because of the low cost of the implant, the ease of its adjustment to the shape of the defect, and the short time of preparation.

Virtual poster abstracts screening for pre-eclampsia and the use of low dose aspirin (ASA) for the prevention of pre-eclampsia. Methods: A brief national-wide online survey was designed to assess the knowledge on pre-eclampsia screening and the usage of ASA for pre-eclampsia prevention. Descriptive statistics were used to characterize the participants' demographic characteristics. Chi-square tests were used to assess differences in health professionals' knowledge on PE screening and ASA usage for PE prevention between provider groups to identify key knowledge gaps. Results: 336 respondents completed the survey: 55.1% OB/GYN,17.0% FP, 14.6%, MFM, 10.0% MW. Guidelines followed: SOGC 57.2%, ACOG 17%, NICE 7.5%, local guidelines 18.2%. Percentage of respondents who prescribe ASA for prevention of pre-eclampsia; 96.7%, prior to 16 weeks' gestation: 96.6%, recommended dose162 mg OD: 38% MFMs 61.2%, OB/GYN 48.6%, FP 45.6%, and MW 38.2%), recommended time of day to take ASA: bedtime 56.5%, recommended gestational age to stop ASA: 36 weeks gestation MFMs (87.8%), Ob/Gyns (71.9%), MWs (67.6%) and FPs (59.6%, p = 0.014). Conclusions: This study provides important information regarding the current practice among obstetric care providers in Canada with regard to pre-eclampsia screening and prevention. While most recommend ASA for the prevention of pre-eclampsia, the screening guidelines followed, ASA dosage and dosage schedules recommended, and timing of stopping of ASA were variable. This baseline knowledge will enable the focused development of educational materials for implementation of PE screening and prevention based on recent high grade evidence regarding predictive screening algorithms and optimal use of ASA for prevention. VP50.05 Histopathological placenta examination as a verification of pre-eclampsia and fetal growth restriction symptoms

For many years, the progress of labour has been traditionally evaluated almost exclusively by transvaginal digital assessment which, by its very nature, is an imprecise and, above all, subjective examination. Appropriate assessment of foetal head station and position in the birth canal is of critical importance for predicting further progress and safe completion of labour by instrumental or surgical intervention. In view of the deficiency of diagnostic methods available in the delivery room, attempts are undertaken to introduce intrapartum ultrasound performed using a transabdominal suprapubic or transperineal approach as a useful diagnostic tool. The examination is performed at the patient's bedside, using a portable ultrasound unit equipped with a convex probe. The method comprises a range of parameters, of which the most common are the angle of progression (AoP), foetal head direction, headperineum distance or midline angle (MLA). Intrapartum sonography yields an array of data to evaluate with a high degree of precision the foetal head position and station in the birth canal. Intrapartum ultrasound may prove a very useful method complementing traditional obstetric examination in a number of clinical situations such as prolonged delivery and lack of certainty as to the way to end the labour. Increasingly, attention is being drawn to the role of the examination in predicting the efficacy of induction of labour, serving as visual biofeedback to increase the effectiveness of maternal pushing or accurately identify the beginning of labour. It has been highlighted that intrapartum ultrasound is easy to use, painless, and reproducible. Also, the method does not require specialist training. Despite promising research results and the development of recommendations on the application of the method, there is still insufficient evidence to elaborate definite algorithms for the interpretation of results, based on which clinical decisions could be made.

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