Introduction: Sepsis is a group of symptoms caused by infection, characterized byorgan dysfunction due to compromised hosts response to infection hence may leadto a life-threatening condition. One of the treatments for sepsis stated in one hour-bundle is the administration of broad-spectrum antibiotics before the culture resultsobtained. Improper use of antibiotics may lead to antibiotic resistance. The purposeof this study was to describe the microbes pattern and their sensitivity to antibioticsin patients with sepsis at Muhammad Hoesin Hospital, Palembang, to contribute toa useful treatment guideline and to provide a reference for further research.Methods: This study was a descriptive observational study with a cross-sectionaldesign using medical record of patients diagnosed with sepsis whose blood cultureresults were positive. This study was conducted at Muhammad Hoesin Hospital,Palembang, from January 2017 to December 2018. The data was processed andanalyzed by univariate analysis using the SPSS 21.0 computer program. Results:The study subjects were predominantly children with Gram-positive bacteria(24.6%) as the most common cause. The most common bacteria observed wereStaphylococcus epidermidis (35.7%), Staphylococcus aureus (27.3%), Staphylococcushaemolyticus (24%), and Staphylococcus hominis (21.4%). Linezolid (100%),nitrofurantoin (100%), and quinupristin/dalfopristin (100%) were found to besensitive to gram-positive bacteria. Conclusion: Gram-positive bacteria were themost common cause of sepsis in Muhammad Hoesin Hospital, Palembang. Thebacteria were sensitive to linezolid, nitrofurantoin, and quinupristin/ dalfopristin.