Background: Bone augmentation is a subject of intensive investigation in regenerative bone medicine and constitutes a clinical situation in which autogenous bone grafts or synthetic materials are used to aid new bone formation. Method: Based on a non-critical defect, Co-Cr barrier membranes were placed on six adult Fauve de Bourgogne rabbits, divided into two groups: whole blood and PRP. Three densitometric controls were performed during the experiment. The animals were euthanized at 30, 45, 60, and 110 days. The presence of newly formed bone was observed. Samples for histological studies were taken from the augmentation center. Results: External and internal bone tissue augmentation was observed in almost all cases. Significant differences between PRP- and whole blood–stimulated bone augmentation were not observed. At 60 days, bones with PRP presented higher angiogenesis, which may indicate more proliferation and cellular activity. Conclusion: PRP activates the bone regeneration process under optimized conditions by stimulation of osteoblast proliferation after six weeks, when a significant difference in cellular activity was observed. Membranes could stimulate bone augmentation at the site of placement and in the surrounding areas.

Photofunctionalization of implant materials with ultraviolet (UV) radiation have been subject of study in the last two decades, and previous research on CoCrMo discs have showed good results in terms of bioactivity and the findings of apatite-like crystals in vitro. In the current study, CoCrMo domes were photofunctionalized with UV radia

Cr-Co-Mo (ASTM F75) alloy has been used in the medical environment, but its use as a rigid barrier membrane for supporting bone augmentation therapies has not been extensively investigated. In the present study, Cr-Co-Mo membranes of different heights were placed in New Zealand white, male rabbit tibiae to assess the quality and volume of new bone formation, without the use of additional factors. Animals were euthanized at 20, 30, 40, and 60 days. Bone formation was observed in all of the cases, although the tibiae implanted with the standard membranes reached an augmentation of bone volume that agreed with the density values over the timecourse. In all cases, plasmatic exudate was found under the membrane and in contact with the new bone. Histological analysis indicated the presence of a large number of chondroblasts adjacent to the inner membrane surface in the first stages, and osteoblasts and osteocytes were observed under them. The bone formation was appositional. The Cr-Co-Mo alloy provides a scaffold with an adequate microenvironment for vertical bone volume augmentation, and the physical dimensions and disposition of the membrane itself influence the new bone formation.

Abstract-Titanium is the gold standard material in oral implantology due to its convenient properties: biocompatibility, low density, high resistance, high rigidity, excellent bone attachment and biological stability. Many researchers have proved that it is possible to improve the implant fixation and to reduce osseointegation time through surface treatments, which lead to the development of an adequate topography. In this study, discs of commercially pure titanium, grade II were subjected to sand blasting using Al 2 O 3 particles, acid etching using HF and HCl/H 2 SO 4 solutions and 600°C heat treatment during one hour. To determine in vitro behavior, samples were submerged in simulated body fluid. The obtained surface characterization was observed by scanning electron microscopy and electron probe microanalyzer. Results showed titanium as the only component of the treated surfaces, which proves acidic treatment effectiveness and an elevated decontamination; moreover, this treatment achieved a submicrometric topography that may influence on clot retention and bone matrix formation. Heat treatment samples showed a coating of crystalline agglomerations, whose elemental composition was calcium and phosphorus, apatitic kind, which follows the rough morphology of substrate.