In today’s modern world the chemical induced hepatotoxicity is one huge threat to human life, even the drugs which have easy accessibility and availability are also produces side effects, when they are used irrationally, so the need for antidote from herbal industry is a common factor. Bauhinia tomentosa Linn belongs to fabaceae, considered as one such potential agent which constitutes wide range of chemical compounds which has therapeutic as well as antidote effect. In this study Bahuinia tomentosa Linn was extracted with ethyl alcohol and the prepared ethanolic extract was evaluated for its hepato protective effect against Acetaminophen induced hepato toxicity in albino mice. The biochemical estimation, histo pathological studies are served as index for the assessment of hepatoprotective activity. Modification in body and liver weight, proteins, levels of biomarkers, antioxidant enzymes along with histopatological variations of extract treated groups were compared with standard hepatoprotective drug silymarin. Marked hepatoprotective activity was noticed in extract treated groups in dose dependent manner. The study results revealed the antihepatato toxic effect of Bauhinia tomentosa Linn and recommended as an excellent natural source of drug in the treatment of acetaminophen induced hepatotoxicity.