This study aimed to verify the occurrence of Dioctophyme renale in stray dogs in the city of Pelotas, in the state of Rio Grande do Sul, Brazil. The Laboratório Regional de Diagnóstico of the Universidade Federal de Pelotas received 146 wandering dogs for necropsy, sent by the City Hall of Pelotas from March 2012 to January 2020. Among the necropsied animals, seventeen dogs (11.64%) were diagnosed with dioctophymosis. Among these dogs, 11 were parasitized with one specimen in the right kidney, two dogs presented two specimens in the right kidney, and in other two dogs, the parasites were in the abdominal cavity. In one dog, two parasites were found in the left kidney; in another dog, both kidneys were parasitized, with two parasites in the right kidney and one in the left kidney. The data obtained in this study showed that the occurrence of D. renale in stray dogs in the city of Pelotas is high, and D. renale mainly parasitizes the right kidney.

O patologista veterinário exerce papel fundamental na medicina veterinária forense. As lesões encontradas e a condição que o animal se encontra podem ser resultantes de atos intencional e a traumatologia forense é importante ferramenta para a identificação de lesões. O traumatismo cranioencefálico (TCE) caracterizam-se por danos físicos ao tecido cerebral. O objetivo deste trabalho é descrever os casos de necropsias com finalidade forense de animais que sofreram trauma cranioencefálico como principal processo de óbito. Através do estudo retrospectivo da casuística do SOVET – UFPel, foram selecionadas necropsias com finalidade forense e histórico de suspeita de trauma cranioencefálico no período 11 anos. Das 14 necropsias forenses realizadas no período, 5 cães apresentavam histórico ou suspeita de trauma cranioencefálico. Em sua maioria eram cães de raça definida e fêmeas. A fratura de osso parietal foi a lesão mais encontrada. Também foram observados fratura de osso occiptal e lesões superficiais em osso frontal. Três dos cinco animais havia suspeita de agressão por pessoas do convívio, caracterizando maus-tratos. Dois dos cinco cadáveres estudados foram exumados, reforçando a realização da necropsia forense como ferramenta essencial para elucidação da causa da morte, principalmente em casos de agressão. Conclui-se lesões de osso parietal foram as mais frequentes e a exumação se faz necessária para elucidar os casos de suspeita de maus tratos. A necropsia forense é essencial para registrar adequadamente as alterações encontradas e estabelecer o nexo causal em casos forenses.

Listeria monocytogenes is a bacterium that infect humans and animals and causes a zoonotic disease characterized by encephalitis, septicemia or abortion. In addition, listeriosis leads to significant economic losses due to animal death and sacrifice. This research compared the technique of immunofluorescence (IF) and immunohistochemistry (IHC) for the diagnosis of L. monocytogenes in formalin-fixed and paraffin-embedded (FFPE) tissues. A total of 30 tissue blocks from 15 animals with history and/or lesions compatible with listeriosis were selected. For both IHC and IF, the same diluted (1:200) polyclonal primary antibody was used against L. monocytogenes serotypes 1 and 4. For IHC, a polymer secondary antibody conjugated to peroxidase (HRP) was used. For IF, samples were incubated with a fluorescein-labeled anti-rabbit IgG secondary antibody. Each sample was classified according to the presence and percentage of immunolabeling area. From 30 samples, 10 were positive at least for one technique, whereas eight samples were positive for both IHC and IF with similar score. There was strong immunolabeling in tissue samples from bovines experimentally infected with L. monocytogenes ATCC 7644, as well as in nervous tissues from naturally infected ruminants. Additionally, IF did not show any difference in sensitivity when compared to IHC. Using processed biological materials for IF, instead of fresh tissues, is a quite unique technique, since there are few protocols described. Therefore, this study demonstrated that both techniques are efficient to detect L. monocytogenes in FFPE tissues.