The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.

ABSTRACT. The present work determined the effects of water with unbalanced calcium hardness/total alkalinity (CH/TA) ratio on Nile tilapia growth performance. Two unbalanced CH/TA ratios were tested for 8 weeks using one indoor system with 30 circular polyethylene 100 L tanks and one outdoor system with 36 circular polyethylene 250 L tanks. The factors and their specific levels tested were the total alkalinity of water (TA; 50 and 100 mg L -1 CaCO 3 ) and the CH/TA ratio of water (CH/TA; 1/2, 1/1 and 5/1). In the indoor system, the 5/1 CH/TA ratio tanks had higher total ammonia nitrogen (TAN) than the 1/2 or 1/1 CH/TA ratio tanks. In the outdoor system, higher concentrations of TAN were found in the 1/2 CH/TA ratio tanks. The soil of the 5/1 CH/TA ratio indoor tanks had higher concentrations of organic carbon than the 1/2 or 1/1 CH/TA ratio tanks. The detrimental effects of the 5/1 CH/TA ratio on fish growth performance were stronger than those associated with the 1/2 CH/TA ratio. A significantly better food conversion ratio was observed in the 1/1 CH/TA ratio outdoor tanks regardless of the TA level of the water. Desequilíbrios da relação dureza/alcalinidade da água e desempenho produtivo da tilápia do Nilo RESUMO. O presente estudo determinou os efeitos de diferentes relações dureza cálcica/alcalinidade total (DC/AT) da água de cultivo sobre o desempenho produtivo da tilápia do Nilo. Duas relações DC/AT foram testadas durante oito semanas em dois sistemas de cultivo: interno, com a utilização de 30 tanques de 100 L e externo, com 36 tanques de 250 L. As variáveis testadas foram a alcalinidade total da água (AT; 50 e 100 mg L -1 CaCO 3 ) e a relação DC/AT da água (1/2, 1/1 e 5/1). No sistema interno, os tanques com relação DC/AT de 5/1 apresentaram maior concentração de nitrogênio amoniacal total (NAT) que os tanques com relações DC/AT de 1/2 ou 1/1. No sistema externo, as maiores concentrações de NAT foram observadas nos tanques com relação DC/AT de 1/2. O solo dos tanques internos com relação DC/AT de 5/1 apresentaram maiores concentrações de carbono orgânico. Os efeitos deletérios da relação DC/AT de 5/1 sobre o desempenho zootécnico dos peixes cultivados foram maiores que aqueles associados à relação DC/AT de 1/2. Fator de conversão alimentar significativamente melhor foi observado nos tanques externos com relação DC/AT de 1/1, independentemente do nível de alcalinidade total da água.Palavras-chave: piscicultura, qualidade de água, Oreochromis nilocitus.

The present work determined the effects of water with unbalanced calcium hardness/total alkalinity (CH/TA) ratio on Nile tilapia growth performance. Two unbalanced CH/TA ratios were tested for 8 weeks using one indoor system with 30 circular polyethylene 100 L tanks and one outdoor system with 36 circular polyethylene 250 L tanks. The factors and their specific levels tested were the total alkalinity of water (TA; 50 and 100 mg L-1 CaCO 3) and the CH/TA ratio of water (CH/TA; 1/2, 1/1 and 5/1). In the indoor system, the 5/1 CH/TA ratio tanks had higher total ammonia nitrogen (TAN) than the 1/2 or 1/1 CH/TA ratio tanks. In the outdoor system, higher concentrations of TAN were found in the 1/2 CH/TA ratio tanks. The soil of the 5/1 CH/TA ratio indoor tanks had higher concentrations of organic carbon than the 1/2 or 1/1 CH/TA ratio tanks. The detrimental effects of the 5/1 CH/TA ratio on fish growth performance were stronger than those associated with the 1/2 CH/TA ratio. A significantly better food conversion ratio was observed in the 1/1 CH/TA ratio outdoor tanks regardless of the TA level of the water.

O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

No abstract