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The use of magnetic brain stimulation has been growing in clinical practice, although the mechanism by which it acts on cells has yet to be elucidated. In order to evaluate morphological and biochemical responses, we submitted astrocytes obtained from the cerebral cortex of Wistar rats to static magnetic stimulation (EME) of 305mT for 5, 15 and 30 min daily for 7 consecutive days. The magnetic stimulus did not affect the astrocyte morphology, but it caused an increase in sulforodamine B in the cells stimulated for 5 and 15 min, as well as, in the production of reactive oxygen species (ROS) only in the time of 5 min daily compared to the control group. In longer stimulation times, such as 30 min daily, they did not show to change any of the evaluated parameters. Our results, although preliminary, demonstrate that in shorter times of stimulation there is an increase in cell density and ROSs, suggesting that with the increase in stimulation time astrocytes may adapt to the insult. However, evaluation of other parameters is necessary to support these findings.