Objective: To analyze the adherence to hand hygiene by the nursing team. Methods: Observational study developed with 85 nursing professionals in the months of September and October of 2018. A script recommended by the National Health Surveillance Agency (ANVISA) and a validated checklist was used. Results: Regarding the characterization of the technique and hand hygiene situation, it was observed that 100% of the nursing assistants presented short nails, performed the nail cleaning, the tips of the fingers and the wrists, but no auxiliary carried out the hygiene of the hands when entering the unit. All the evaluated professionals used the liquid soap to hygienize the hands. The time spent for hygiene was 10-20 seconds between all the categories studied. Conclusion: No category obtained 100% correct hand hygiene as recommended by ANVISA. Interventions are necessary to obtain full compliance by nursing professionals.