The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multispecies biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (10 6 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.
Objective: Radiomorphometric indices (RI) vary according to gender and age. The objective of this study was to assess bone quality in men and women, using tomographic images Methods: A total of 78 Cone Beam Computed Tomography (CBCT) images of female and male patients were analyzed. The images were obtained from LAPI-FOUSP database, São Paulo, Brazil, and the Mental Index, which is a quantitative index, was evaluated on those images. The images were divided into 2 groups: Group M – Men and Group W – Women and evaluated by one examiner in two different times (T1 and T2). The data was submitted to a statistical analysis with a 95% level of significance (p <0.05) Results: The ICC tests showed an average to good intra-examiner agreement. The age averages were: 48.46 ± 15.3 for group W and 53.87 ± 13.09 for group M. On the MI evaluation, statistically significant differences were observed between the two groups Conclusion: We concluded that MI can be used to determine sexual dimorphism, once the thickness of the mandible cortex in women is smaller than the thickness found in men.
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