Bioremediation concerns the use of plants and microorganisms or their parts, for the decontamination and recuperation of polluted areas. The improvement of the bioremediation techniques is possible due to the last decade advances of the microbiological and biotechnological knowledge and methods. This study aimed to shortly review and discuss the bioremediation of mining areas contaminated with heavy metals focusing in some microbiological and biotechnological techniques. Strategies considered to be important on further studies are also presented. Vieira and Stefenon; JAMB, 4(1): 1-10, 2017; Article no.JAMB.33782 2 Mini-review Article

The indoleacetic acid produced by symbiotic bacteria is an important phytohormone signaling microbe-plant interaction, being therefore essential for rhizoremediation. In this study, the effect of different concentrations of copper ions on the bacterial growth and indoleacetic acid production was investigated in two strains of Mesorhizobium loti in in vitro conditions, aiming to determine critical concentrations of this heavy metal for rhizoremediation of contaminated soils using this bacterium. The experiment consisted on a control culture without copper and three treatments supplemented with 10 mg.L -1 , 20 mg.L -1 or 50 mg.L -1 of CuSO 4 . For both strains, the growth stopped after 48h and no significant difference was observed across treatments. The production of indoleacetic acid by the control treatment without copper was significantly higher in comparison to the copper-containing treatments. Mesorhizobium loti SEMIA806 and SEMIA816 are resistant to up to 50 mg.L -1 of CuSO 4 in the culture medium, presenting effective growth. The synthesis of indoleacetic acid was strongly reduced but not excluded by ions copper in the medium. So, it is expected that environmental copper found in the soil up to the concentration of 50 mg.L -1 will not preclude the symbiotic interaction between M. loti and leguminous host plant in rhizoremediation enterprises.

Greywater is a potential source for producing water that can be reused for non-potable purposes. The use of nature-based solutions (NBS) for greywater treatment can contribute to the mitigation of heat and low relative humidity in urban areas. Thus, the goal of this technical note is to present the performance of constructed wetlands, a NBS, with respect to the main parameters of national and international standards considering water reuse, and to assess the suitability of the treated effluent for different reuse purposes. Design examples and a flowchart are presented to support the decision-making process, addressing aspects from the characterization of greywater until choosing an application for the recovered water. In this way, we expect to provide a starting point for enabling an increased reuse of treated greywater for non-potable purposes in Brazil, assisting designers, as well as technicians from regulatory bodies, in the design and evaluation of treatment systems using a simple and promising ecotechnology.

Em trabalhos seguintes, é recomendável a continuidade do projeto desenvolvido, visando monitorar os espécimes plantados e compreender a percepção ambiental dos discentes, bem como executar o plantio de novas mudas em outras áreas da escola. Além disso, é importante analisar os índices arbóreos de escolas públicas do município em estudo, priorizando novos projetos de plantio, principalmente com espécies nativas, em escolas com déficit de arborização. AgradecimentosÀ Escola Estadual Senador Francisco Pessoa de Queiroz por permitir a execução do projeto e ao viveiro de mudas do IFPE -campus Vitória de Santo Antão pela doação das espécies para plantio.

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