Objective: to analyze the biosafety measures adopted by nursing undergraduates in daily activities in spaces of laboratory practices.Method: descriptive, qualitative analysis study performed at a Higher Education Institution in the period from April to June 2018, with 30 students. For the analyses of interviews, Collective Subject Discourse was utilized. Results: as for Personal Protective Equipment (PPE) usage, 24% used gloves, followed by white coat and mask, with 19% and 18% each, respectively. Referring to moments of Hand Hygiene (HH) during the laboratory practices, 32% performed the hygiene before and 26% after the practice. It is observed that the scholars’ knowledge about biosafety is broad but limited to PPE usage. Conclusion: it is verified that the use of biosafety measures in this environment is still a challenge, for it be limited to PPE and HH usage.