The objective of this study was to determine the differences in response to distillers dried grains with solubles (DDGS) level under constant nutrient or floating nutrient concentrations. A total of 21 ileal-cannulated gilts (33.1 ± 0.4 kg body weight) were randomly allotted to one of seven dietary treatments in a 3-period incomplete Latin square design (n = 9). Treatments consisted of a 0% DDGS basal diet, plus diets containing 15%, 30%, or 45% DDGS. Diets were formulated using one of two different formulation methods: 1) constant nutrient (CNU) where nutrients were held equal to the basal diet or 2) constant ingredients (CIN) where DDGS were added at the expense of corn and all other ingredients remained constant, so nutrient levels were allowed to “float.” Chromic oxide was added to the diets at 0.5% as an indigestible marker. Increasing the level of DDGS decreased the apparent ileal digestibility (AID) of dry matter (DM), gross energy (GE), starch, dispensable amino acids (AA), and fiber components (P < 0.050). The decrease in the AID of Lys, Met, Thr, and Trp was more pronounced under CNU compared with the CIN formulation method (P < 0.050). The decrease in the AID of hemicellulose was less pronounced under CNU compared with the CIN formulation method (P = 0.045). There was a DDGS level × formulation method interaction for the AID of acid hydrolyzed ether extract (AEE; P = 0.015); for the CNU formulation method, increasing level of DDGS decreased the AID of AEE from 0% to 30% and remained similar from 30% to 45% DDGS, whereas the CIN had no effect on the AID of AEE. Increasing the level of DDGS decreased the apparent total tract digestibility (ATTD) of DM, GE, and fiber components (P < 0.050), except for acid detergent fiber, which was not affected. The decrease in the ATTD of insoluble dietary fiber and total dietary fiber was less pronounced under CNU compared with CIN (P < 0.050). The ATTD of AEE decreased for CNU compared with CIN (P < 0.010). In conclusion, increasing the insoluble fiber level in the form of DDGS decreased the digestibility of most dietary components, including DM, GE, starch, insoluble fiber, and AA. The CNU and CIN formulation methods are equivalent when evaluating the digestibilities of DM, GE, starch, crude protein, and AA (when they were not added in purified synthetic forms). Differences between CNU and CIN formulation methods were detected for the digestibility of insoluble fiber, fat, and essential AA (when added as crystalline AA).
The experimental objective was to determine the role of mean particle size (PS), grinding method, and body weight (BW) category on nutrient, fiber, and energy digestibility of corn. A total of 48 barrows were housed in individual pens and randomly assigned to one of six dietary treatments for 11 d at two BW categories (55 kg and 110 kg). The six treatments consisted of corn ground at three different targeted mean PSs (300, 500, and 700 µm) using either a roller mill or a hammermill. Fecal samples were collected for the last 3 d of each feeding period. Titanium dioxide was used as an indigestible marker. Digestibility data were analyzed as a linear mixed model using the MIXED procedure of SAS. Finishing pigs had greater apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), and N than growing pigs (P = 0.02, P = 0.01, and P <0.01, respectively). The ATTD of DM, GE, and N was similar in pigs fed hammermilled corn across all PS treatments. However, in roller-milled corn, they increased as PS was reduced (P < 0.05). The ATTD of acid-hydrolyzed ether extract (AEE) in growing pigs was similar between corn ground at 700 and 500 µm, but it was increased by further reducing PS to 300 µm (P < 0.05). In finishing pigs, the ATTD of AEE increased as mean PS decreased from 700 to 300 µm (P < 0.05). The ATTD of AEE was similar in hammermilled corn at all three PS treatments. On the other hand, the ATTD of AEE was similar in corn ground in a roller mill to 700 and 500 µm, but it increased when PS was reduced to 300 µm (P < 0.05). In conclusion, reducing PS of corn with a roller mill increased digestibility of energy and nutrients, but there was less effect using a hammermill. It is possible that differences in SD, distribution, chemical composition, and the shape of the particles resulting from the two grinding processes help to explain the different response.
The objective of this study was to determine the effects of lactose (LA) and a prototype Lactobacillus acidophilus fermentation product (FP) on growth performance, diet digestibility, nitrogen (N) balance, and intestinal function of weaned pigs. Twenty-eight newly weaned pigs [approximately 21 d of age; initial body weight (BW) = 5.20 ± 0.15 kg] were housed in metabolism crates and assigned to one of four treatments (n = seven pigs per treatment) corresponding to a 2 × 2 factorial design: with (LA+; 15% inclusion) or without (LA−) LA and with (FP+) or without (FP−) the prototype FP (1 g of FP per kilogram of diet; Diamond V, Cedar Rapids, IA). Feed and water were provided ad libitum. At day 5, pigs were orally given lactulose and mannitol to assess small intestinal permeability. Fecal samples were collected on days 5–9 to determine the apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), and N. Total urine output and fecal samples were collected on days 10–13 to determine N retention. On day 15, all pigs were euthanized to collect intestinal lumen and tissue samples. Data were analyzed for the main effects of LA and FP and their interaction using the MIXED procedure of SAS. Lactose improved average daily feed intake (ADFI; P = 0.017), the ATTD of DM (P = 0.014), the ATTD of GE (P = 0.028), and N retention (P = 0.043) and tended to increase the butyric acid concentration in the colon (P = 0.062). The FP tended to increase the digestibility of N (P = 0.090). Neither LA nor the FP affected intestinal barrier function or inflammation markers. The interaction between LA and FP affected intestinal morphology: in the jejunum, pigs fed LA+FP− had increased villus height compared with those fed LA+FP+ and LA−FP−, whereas LA+FP+ was intermediate (interaction P = 0.034). At the terminal ileum, pigs fed LA−FP+ and LA+FP− had increased villus height and villus: crypt compared with those fed LA−FP−, whereas LA+FP+ was intermediate (interaction P = 0.007 and P = 0.007, respectively). In conclusion, the addition of LA brings important nutritional attributes to nursery diets by improving feed intake, digestibility of DM and GE, and the N retention of weaned pigs; however, the functional capacity of LA to improve markers of intestinal function is limited. On the other hand, the FP showed only a mild increase in the digestibility of N but a limited capacity to improve markers of intestinal function.
Rising feed expenditures demand that our industry pursues strategies to lower the cost of production. One option is the adoption of the NE system, although many producers are hesitant to proceed without proof that NE estimates are reliable. The objective of this experiment was to compare the apparent total tract digestibility (ATTD) of energy and nutrients and the N retention (NR) of diets formulated using the NE system with increasing quantities of coproduct ingredients. The 5 dietary treatments included a control corn-soybean meal diet (CTL); the CTL plus 6% each of corn distiller's dried grains with solubles (DDGS), corn germ meal, and wheat middlings and NE equal to the CTL by adding soybean oil (CONS-18); the CONS-18 diet, without oil added, with NE content lower than the CTL (DECL-18); the CTL plus 12% each of corn DDGS, corn germ meal, and wheat middlings and NE equal to the CTL by adding soybean oil (CONS-36); and the CONS-36 diet, without oil added, with NE content lower than the CTL (DECL-36). Diets were formulated for both the growing period (GP; 40 to 70 kg) and the finishing period (FP; 70 to 110 kg). Forty gilts (PIC 337 × C22 or C29; 38.5 ± 0.4 kg initial BW) were randomly assigned to treatment and received feed and water ad libitum (8 pigs per treatment). For the last 13 d of the GP and FP, pigs were transferred to metabolism crates, where 2 total urine and fecal collections (d 4 to 6 and d 11 to 13) were performed. The GP fed diets with coproduct ingredients had lower ATTD of DM, N, and GE than those fed the CTL ( < 0.050). The ATTD of N and GE progressively decreased as coproduct inclusion increased from 0 to 18 to 36% in the FP ( < 0.010). In the GP and FP, there were no differences in ATTD of DM, N, or GE between CONS-18 and DECL-18 or between CONS-36 and DECL-36 ( > 0.050). The NR declined on all coproduct diets in the GP ( = 0.010) and tended to decline in the FP ( = 0.079). There were no differences in NR between CONS-18 and DECL-18 or between CONS-36 and DECL-36 ( > 0.050). In conclusion, digestion of diets containing up to 36% coproducts and formulated using NE resulted in expected DE and ME values; NR of diets with coproducts was lower than that of the simple CTL, which is not related to the accuracy of the energy estimations but rather to other factors such as imbalances in the AA concentrations or to postabsorptive energy metabolism, factors not accounted for by the current energy systems approach.
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