Pressurized membrane used for side-stream MBR process requires fouling control strategy both for normal and abnormal operation conditions for stable operation of the facilities. In this study, 85 m 3 /day of pilot-scale side-stream MBR process was constructed for the evaluation of fouling mitigation by air bubble injection into the membrane module. In addition, fouling phenomena at abnormal operation conditions of low influent and/or loading rate were also investigated. Injection of air bubble was found to be effective in delaying transmembrane pressure (TMP) increase mainly due to scouring effect on the membrane surface, resulting in expanded filtration cycle at a high flux of 40 L/m 2 ･h (LMH). At abnormal operation condition, injection of PACl (53 mg/L as Al) into the bioreactor showed 19% reduction of TMP increase. However, inhibition of nitrifying bacteria by continuous PACl injection was observed from batch experiments. In contrast, injection of powdered activated carbon (PAC, 0.6 g/L) was able to maintain the initial TMP of 0.2 kg/cm 2 for 5 days at the abnormal conditions. It may have been caused from the adsorption of extracellular polymeric substances (EPS), which was known to be excessively released during growth inhibition condition and act as the major foulants in MBR operations.

Amongst sludge reduction strategies, the anaerobic side-stream sludge holding tank (SHT) is of particular interest because it has shown significant sludge reduction efficiency. However, due to the anaerobic and starving environment of the SHT, the release of extracellular polymeric substance (EPS) may be stimulated, and it may hamper the application of the SHT to the membrane bioreactor. In order to investigate the effect of sludge storage on EPS release, sludge samples from a pilot-scale sequencing batch reactor coupled with SHT was incubated in a series of bench-scale SHT reactors for different periods of time (0-24 h). The increase in EPS was not significant until 12 h of incubation (9.3%), while 40.9% of the increase was observed in the sample incubated for 24 h. The rapid increase in EPS concentration after 12 h indicates a greater rate of cell lysis than that with EPS consumption as substrate. Since inducing the initial stage of the endogenous phase within microorganisms is a key factor for the successful operation of the SHT for sludge reduction, the retention time for the SHT should be shorter than the time for the sudden increase in EPS release.

AISP: acid-insoluble polyphosphate; ASP: acid-soluble polyphosphate; BNR: biological nutrients removal; EPS: extracellular polymeric substance; LMH: L/m h; MBR: membrane bioreactor; OST: oxic-settling-anaerobic; PAO: phosphate accumulation organism; PCA: perchloric acid; SBR: sequencing batch reactor; SHT: sludge holding tank; SRT: solids retention time; TN: total nitrogen; TP: total phosphate; WAS: waste activated sludge.

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