We have constructed a vector pCAMBIA1300-CP:CBF3-35S:ICE1 and transformed into Arabidopsis. Results of PCR proved that the target genes had integrated into Arabidopsis genome. Transgenic Arabidopsis showed a bit slow growth, earlier flowering, but normal at other phenotype under 22°C with 8 h daily lights. In vitro low temperature stress tests showed that the transgenic lines were survival while the wild type was nearly dead. The transgenic plants also showed an increased proline content, SOD and POD activities under low temperature stress. The phenotype and physical evidence indicated that expression of CP:CBF3-35S:ICE1 under low temperature enhances the cold tolerance in transgenic plants.

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2, 4-D and picloram were compared for their ability to the induction of somatic embryogenesis in Chinese cassava (Manihot esculenta Crantz) cultivars SC5, SC6, SC7 and SC8. In all four cultivars tested, both 2, 4-D and picloram had the capacity to induce primary somatic embryos from axillary buds. And the two hormones were also suitable for subculture of somatic embryos of three cultivars SC5, SC6 and SC8. However both 2, 4-D and picloram can not keep the activity of somatic embryos of cultivar SC7. For organogenesis, cotyledon matured for 10~15 days were better than others.